Sajany

1. Sajany Joint Stock Company Gas Vortex Gradientless Bioreactor Сreation of highly efficient universal modular biotechnological complexesof anew type based on gas vortex gradientless bioreactors 630090 office 403, building 8, Nikolaeva str., Novosibirsk Tel/fax: +7 (383) 3-33-33-69; 3-33-26-01, 3-30-92-31 e-mail: sajany@bioreactor.ru, sajany@ngs.ru www.bioreactor.ru

2. The factors affecting the vital capacity of cultivated cells and microorganisms metabolites O2 O2 O2 O2 O2 O2 O2 O2 O2 O2 O2 O2 O2 cut strain nutrition cut strain (temperature regime, breath, nutrition, metabolites, mechanical impact)

3. Mechanical mixing Gas vortex mixing

4. Traditional bioreactor: mechanical mixing

5. Gas vortex gradientless bioreactor: gas vortex mixing

6. Characteristics of gas vortexgradientless bioreactors • Extremely low power consumption, i.e. 10-12 times less compared to bioreactors with mechanical stirrer • Cultivation of cells which are poorly reproduced in well-known types of bioreactors • Highsurface mass exchange rate- 6-8 (KL 1/ hour) • Efficient mixing of especiallyviscous fluids • Absence of local overheating areas affecting the enzymes activity • Constant operational parameters when filled with a suspension by 15%-90% of the vessel volume • Gas vortex serves as an efficient foam breaker • Bioreactor’s hydrodynamics, practically, does not depend on the liquid level inside. The bioreactor is easy to scale-up

7. SomeCultivation Results (Without Medium Substitution) Besides, chicken embryo fibroblasts, VERO, hybrid A4С5 cells (a hybrid of porcine kidney cell and lymphocyte), human thymus cells (T-5) and Aristolochia manshunensis cells, Aspergillus awamori, Fusarium moniliforme and many others were successfully cultivated.

8. Important advantagesof gas vortexgradientless bioreactors • Universality – capability to operate successfully practically on all types of cells (including embryonic and hybrid ones) and microorganisms • Fullrepeatability(scalability) of laboratory results at industrial application of developments • Higheconomicefficiency in commercial use

9. Industrial implementation of innovative products and technologies !! ! New product New product New product Gradientlessbioreactor ? ?? Traditionalbioreactor

10. Application of gas-vortex bioreactors for emergency responce Global warming Technogenic impact Bioterrorism Developments of required vaccines Express manufacturing of any type vaccines in sufficient amounts using gas-vortex bioreactors Appearance of diseases unknown before Epidemics, pandemics Emergency eliminations

11. Conception ofhighly efficient universal modular biotechnological enterprises of a new type • Highly efficient universal modular biotechnological enterprise of a new type consists of: • fixed highly economical “head” block of the universal gas vortex gradientless bioreactors •   variable modular “tail” block responsible for the processing of biological products • Modules of the “tail” block can be modified and enlarged depending on the processed biological product which is got from block of gas vortex gradientless bioreactors

12. Universal highly efficient biotechnological modular production facilities 10 L 10 L 750 L 10 L 300 L 100 L 30 L 10 L 750 L 10 L 30 L 100 L 300L Fermentative unit withgas-vortex bioreactors Fermentative unit withtraditional bioreactors

13. Factors that highlight high profitability of the new production facility • Minor initial investments as a results of • Reduction of the number of bioreactors in the technological chain • Making communication pipelines simpler and shorter • Reduction of a total production costs (electric power, steam, detergents, area, payment schedule etc.) • Universality of the facilities, i.e. feasibility of successful operation with producers of different types • Financial stability of the enterprise as a result of rapid and low-investment implementation of technologies for manufacture of new biotechnological products • Technological effectiveness • Augmentation of the biomass yield • Reduction of losses during cultivation

14. Field of Application • Production of medical products (including products using very sensitiveembryonic, hybrid and other cells); • Production of a broad range of microbiological preparations for agriculture and veterinary medicine; • Production of polysaccharides and oil destructors for oil-producing industry; • Manufacturing of products for food industry (treacle, glucose syrups,ferments, food supplements and etc.); • Production of surface-active agents and ferments for chemical industry; • Carrying out research efforts of different types.

15. Promptly-built modular biotechnological (pharmaceutical) industrial complexes of a new type Broad application of gas-vortex bioreactors and the creation of biotechnological industrial complexes of the new type on their basis is one of the breakthrough trends in the field of industrial biotechnology.

16. Gas Vortex Gradientless Bioreactor is an apparatus of new generation having no analogues in the whole worldin terms of the method of mixing patents of USA, Japan and 6 European countries In III Moscow International Congress «BIOTECHNOLOGY: STATE OF THE ART AND PROSPECTS OF DEVELOPMENT» Gas Vortex Gradientless Bioreactor became the winner in the nomination “Equipment” and was awarded gold medal at BIOTECH WORLD’ 2005 International Exhibition. Gas-Vortex Gradientless Bioreactor is acknowledgedby experts to be one of the most promising creations in the sphere of high technologies in Russia in 2004.

17. SajanyJoint Stock Company Thank you 630090 office 403, Building 8, Nikolaeva str., Novosibirsk Tel/fax: +7 (383) 3-33-33-69; 3-33-26-01, 3-30-92-31 e-mail: sajany@bioreactor.ru, sajany@ngs.ru www.bioreactor.ru