1 / 17

Exploring Enzyme Activity: Hypotheses, Observations, and Experiments

Conduct experiments with water, vinegar, and white powder to study enzyme activity, observe reactions, and analyze enzyme-substrate interactions.

kiet
Download Presentation

Exploring Enzyme Activity: Hypotheses, Observations, and Experiments

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Enzyme Activity • C: Collect two beakers and a pair of goggles for each person in your group. • D: Collect white powder and vinegar from me. • A&B: Clean-up Crew • Hypothesis: create two hypothesis using two variables, water and vinegar, and adding each substance to the white powder.

  2. Once you have created your hypothesis place half of the powder in one beaker and the other half in the other beaker. • Place water in one beaker--) observe what happens • Place vinegar in the other beaker observe what happens • Write all you observe. • What happened in the beakers? Use science words • Why did this happen? Again use science terms

  3. Warm-Up • What caused the fizzing of the baking soda and vinegar? • What are the two parts of a chemical reaction?

  4. Enzymes

  5. What Are Enzymes? • Most enzymes are Proteins • Act as Catalyst to accelerate a reaction • Not permanently changed in the process

  6. Enzymes • Are specific for what they will catalyze • AreReusable • End in –ase -Sucrase -Lactase -Maltase

  7. How do enzymes Work? Enzymes work by weakening bonds which lowers activation energy

  8. Enzymes Without Enzyme With Enzyme Free Energy Free energy of activation Reactants Products Progress of the reaction

  9. Enzyme Toothpickase Activity • Make 4 stacks of 40 toothpicks • We are the enzyme toothpickase • Your thumb and index finger are catalase You are given 10seconds, 30seconds, 60 seconds, 90seconds. I will call time after each segment and you will move on to the next pile of toothpicks. You cannot go onto a new pile until time is up! You can only break the toothpicks that have not been broken. YOU DO NOT LOOK AT THE PILES AS YOU PICK UP THE TOOTHPICKS.

  10. Questions • Count each pile: How many toothpicks did you metabolize? TIME (sec)# METABOLIZED 10 30 60 90 • What happened to catalase when it started running out of toothpicks?

  11. Enzyme-Substrate Complex Enzyme The substance (reactant) an enzyme acts on is the substrate Joins Substrate

  12. Active Site Enzyme • A restricted regionof an enzyme molecule which binds to the substrate. Active Site Substrate

  13. Induced Fit • A change in the shape of an enzyme’s active site • Induced by the substrate

  14. Induced Fit Active Site substrate Enzyme induced fit • A change in the configuration of an enzyme’s active site (H+ and ionic bonds are involved). • Induced by the substrate.

  15. What Affects Enzyme Activity? 1. Extreme Temperature are the most dangerous - high tempsmay denature (unfold) the enzyme. 2. pH (most like 6 - 8 pH near neutral)

  16. 1. Environmental Conditions 1. Extreme Temperature are the most dangerous - high tempsmay denature (unfold) the enzyme. 2. pH (most like 6 - 8 pH near neutral) 3. Ionic concentration (salt ions)

More Related