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Coomassie. a -PrP. a -GFP. 124-230. 124-230. 124-230. 124stop. 124stop. 124stop. Vector. CyPrP. CyPrP. CyPrP. Vector. Vector. *. *. CyPrP. −250−. −150−. *. *. 124stop. *. −100−. 124-230. −75−. −50−. −37−. −25−. 100. N2a lysate. 80. Mouse brain. 60. 40. 20.

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  1. Coomassie a-PrP a-GFP 124-230 124-230 124-230 124stop 124stop 124stop Vector CyPrP CyPrP CyPrP Vector Vector * * CyPrP −250− −150− * * 124stop * −100− 124-230 −75− −50− −37− −25−

  2. 100 N2a lysate 80 Mouse brain 60 40 20 Frequency, % 10 0 0.00- 4.00 4.01- 8.00 8.01- 12.00 12.01- 16.00 16.01- 20.00 20.01- 24.00 24.01- 28.00 28.01- 32.00 32.01- 36.00 Raw levels

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