1 / 2

Methods for studying Adipocyte Lipolysis

Methods for studying Adipocyte Lipolysis. Whole-Body Lipolysis Ra of glycerol in the bloodstream Measuring the dilution of tracer-labeled glycerol in plasma (isotopic dilution) Has some possible inconsistencies Regional Lipolysis

kimberley-edwards
Download Presentation

Methods for studying Adipocyte Lipolysis

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Methods for studying Adipocyte Lipolysis • Whole-Body Lipolysis • Ra of glycerol in the bloodstream • Measuring the dilution of tracer-labeled glycerol in plasma (isotopic dilution) • Has some possible inconsistencies • Regional Lipolysis • Lipolytic activity can be assessed in individual tissues using arteriovenous methods • Evaluation of the balance between delivery and release of glycerol across adipose tissue bed. • Done by measuring arterial & venous glycerol concentrations and adipose tissue blood flow (ATFB) • Methods were arterial and venous catheterization and microdialysis probes. Schematic diagram of a microdialysis probe and the method used to collect samples

  2. More Measurement Techniques • Measurements of hormones, metabolite concentrations and blood lactate concentrations were measured by Goto via different methods • Epi & Ne measured with high performance liquid chromatorograph kits • Serum GH measured with radioimmunossay kits • Serum Insulin measured with Enzymic immunoassay • Serum FFA & Ketone Body measured with Enzymatic methods • Serum Glycerol measured with Enzymatic colorimetric • Lactate measured by pricking a fingertip for a blood sample

More Related