Senior Design P09052 Molecular Imaging System Upgrade

1. Senior Design P09052 Molecular Imaging System Upgrade Ben McGee Brandon Luquette Patricia Heneka Dien Nguyen Aaron Phipps

2. Project Description • To develop a research prototype of a clinical system capable of performing depth-resolved optical molecular imaging of a stationary human hand • Integration of Qioptiq Linos’s Optigrid with existing Carestream molecular imaging platform. • Prototype must be functional and flexible enough for Carestream to investigate for commercialization

3. Existing Platform

4. Proposed Modifications

5. Optigrid Functionality  A portion of the OptiGrid at 200x y z x • Motors provide 120o phase shifts and focus • Grid moves on the x-axis 11.11 um to complete a phase change • Improves Clarity • Grid moves 2 mm on the z-axis to focus image • 30 line pair/mm on a 10x10 mm square • Lines spatially modulate the illumination

6. Design Objective • Projection of the Optigrid at an angle while maintaining focus

7. Mechanical Design Solution

8. 30º 17 in Implemented Mechanical Design Lens Fixture Optigrid Light Source

9. System Analysis and Calibration • Characterization • Capable of projecting grid at spatial frequencies ranging from 0.8 – 1.4 line pair/mm • Grid starts with spatial frequency of 30 line pairs/mm • Grid must be uniform and undistorted over image plane • Calibration • Developed a system specific procedure to provide repeatable phase movements • Processing • Depth discrimination proof of concept using depth of modulation analysis

10. System Characterization .8 line pair/mm • Projected at a 30o angle • Steepest angle capable of the system • The system was set to a zoom of 40 pixels/mm • Magnified using Computar C mount lens • Different Lenses provide different spatial frequency ranges • Captured with a blue excitation filter and a green emission filter 1.4 line pair/mm Calibration images taken with non glossy white target on platen

11. Calibration • Coarse Calibration • Capture images in a general range of 30, 55 and 90 V • Calculate phase at each point • Determine approximate voltages for 120ophase shifts.

12. Demodulation • Images are combined to eliminate the projected frequency using • Incorrectly phase shifted images have residual lines Misaligned image Properly aligned image

13. Processing • Phase shifted images were captured of the phantom block • Fluorescent sheets were inserted between polycarbonate sheets • The block was imaged at two spatial frequencies

14. Depth Discrimination By assessing the depth of modulation at each pixel of the image, and monitoring its value at each phase shift, information about the depth of the pixel can be assessed. Intensity Intensity Horizontal Spatial Coordinate Horizontal Spatial Coordinate Intensity Intensity Phase Phase

15. Depth Discrimination • Squared error away from the mean at each point is used to evaluate the depth at each point. MSE Surface plot of MSE for each pixel Image of Phantom Block

16. Recommendations • Projection Angle • A shorter rail will result in a steeper projection angle which would reduce distortion P/N: NT54-928 P/N: NT54-929 Edmund Technical Drawings

17. Recommendations • Automation • The calibration process is very time consuming and prone to human error. • Timing is very critical to achieving repeatable phase shifts in the Optigrid. • Calibration Procedure Modifications • Warm up routine • Increase the number of phase shifts

18. Thanks! • Dr. Gilbert Feke – Carestream Health • Linda Antos – Qioptiq Linos Imaging Solutions • Dr. Daniel Phillips – RIT EE Faculty • Dr. Maria Helguera – RIT Imaging Science Faculty • Carestream Health – Primary Sponsor • Qioptiq Linos Imaging Solutions - Sponsor • Dr. Robert Doolittle – RIT Biomedical Science Faculty • Heather Drake – Medical Science Faculty