120 likes | 291 Views
E. P. M. PemPlant Co. April 26, 2007 Update Presentation. Maricela Delgadillo Kay Furman Yushan Kim. Wear Testing: ASTM Standards. ASTM G 99 Equipment: Variable speed motor Disk and pin setup Sample stand Gloved probe. Wear Testing. Tip Construction. Gloved Tip.
E N D
E P M PemPlant Co. April 26, 2007 Update Presentation Maricela Delgadillo Kay Furman Yushan Kim
Wear Testing: ASTM Standards • ASTM G 99 • Equipment: • Variable speed motor • Disk and pin setup • Sample stand • Gloved probe PemPlant Co.
Wear Testing Tip Construction Gloved Tip Calibrated Weight Bouncy Ball Full Apparatus PemPlant Co.
Wear Testing Methods • Probe mass: 61 g • Worn for 10 min at 2 rpm 10 swipes
Wear Testing: Assessment • Profilometry conducted on Tencor P-10 surface profiler • 2 μm radius diamond stylus scans at 6.0 mg • 590 μm scan on a scratched film: PemPlant Co.
Molecular Force Probe • Young’s modulus obtained by linear regression of Hertzian contact model • Our films: 123 KPa • Literature value for identical film: ~200 Kpa PAA/PAH 2.0/2.0 on Ti PemPlant Co.
Bacteria Experiment: Methods • Day 1 • Prepare media and inoculation • Day 2 • Sterilize • Prepare bacteria solution in sterile water • Seed samples for timepoints • Remove samples from solution, place agar gel on top • Incubate ~24 h, hope they grow • Day 3 • Remove from incubation, refrigerate PemPlant Co.
Glass PEM Results PemPlant Co. 10
Roughness effects Bacteria settle in cracks on bare Ti PEMs even prevent colony formation on cracks Ti PEM Results Bare Ti 2.0/2.0 3.0/3.0 PemPlant Co.
Ti PEM Bacteria • Bacteria are not viable over time periods due to lack of nutrients • 8 h experiment would require glucose in solution PemPlant Co.
Future Steps • Stability testing in PBS or water • Biofilm-forming Staph epi strain • More thorough MFP PemPlant Co.