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Threshold dependent BMP Signaling: A Conserved Mechanism for Patterning the Ectoderm

Threshold dependent BMP Signaling: A Conserved Mechanism for Patterning the Ectoderm. Ethan Bier. Section of Cell and Developmental Biology, UCSD. dpp. dpp. “sog”. sog. snail. Epi. Neuro. Neuro. Dorsal. Meso. Epidermal Genes. Neuronal Genes. B. r. k. Neuronal Genes.

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Threshold dependent BMP Signaling: A Conserved Mechanism for Patterning the Ectoderm

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  1. Threshold dependent BMP Signaling: A Conserved Mechanism for Patterning the Ectoderm Ethan Bier Section of Cell and Developmental Biology, UCSD

  2. dpp dpp “sog” sog snail Epi Neuro Neuro Dorsal Meso

  3. Epidermal Genes Neuronal Genes B r k Neuronal Genes Mesodermal Genes = M e s o d e r m = N e u r o e c t o d e r m = N o n - N e u r a l E c t o d e r m Sog blocks BMP invasion of the neuroectoderm BMP Sog Dorsal

  4. Neuronal Genes Epidermal Genes Neuronal Genes B r k Sog Mesodermal Genes = M e s o d e r m = N e u r o e c t o d e r m = N o n - N e u r a l E c t o d e r m Neural Genes are Repressed By BMP Signaling X Dpp

  5. = M e s o d e r m = N e u r o e c t o d e r m = N o n - N e u r a l E c t o d e r m Neural Genes are Repressed By BMP Signaling Neuronal Genes Mesodermal Genes

  6. dpp- Neural Genes are Repressed By BMP Signaling wt l’sc tkv

  7. Neuronal Genes Neuronal Genes Mesodermal Genes = M e s o d e r m = N e u r o e c t o d e r m = N o n - N e u r a l E c t o d e r m Dpp Invades the Neuroectoderm of sog- Mutants Epidermal Genes Dpp X Sog

  8. Neuronal Genes = M e s o d e r m = N e u r o e c t o d e r m = N o n - N e u r a l E c t o d e r m Dpp Invades the Neuroectoderm of sog- Mutants Epidermal Genes Dpp Mesodermal Genes

  9. sog-; Dpdpp Dpp Invades the Neuroectoderm of sog- Mutants wt

  10. The Default state of Ectoderm is Neural

  11. Non-neural genes Dpp Neural genes B r k Sog Subdivision of the neuroectoderm Dorsal

  12. Non-neural genes Dpp Neural genes Msh Neuronal genes Ind Vnd Subdivision of the neuroectoderm Dorsal

  13. Dpp dpp msh ind vnd Dorsal BMPs Msx Gsh Nkx2.2 Mouse Neural Tube Sonic Hh Patterning the neuroectoderm in flies and mice DrosophilaNeuroectoderm

  14. Ventral dominance Ventral dominant repression D msh ind vnd V

  15. 2 “ind” st2-dpp Generating lateralized embryos ECT 3 NE 2 1 MES Mid-lateralized Embryos -> Uniform “ind” levels of Dorsal

  16. dpp msh ind vnd Gene expression in lateralized embryos Mid-Lateralized Embryos Dorsal

  17. sog- sog dpp pMAD Localized Dpp activity along the A/P axis sog+ sog dpp pMAD

  18. merge dpp Subdivision of the neuroectoderm

  19. ECT 3 Brk Dpp 2 NE 1 MES st2-brk st2-brk Gene expression borders shift in BMP- cells

  20. Dosage dependent BMP-repression in vertebrates

  21. BMPs can pattern lateralized vertebrate embryos

  22. VertebrateNeuroectoderm Dpp BMP4 msh Msx1/2 Sog Chd ind Gsh/Pax6 vnd Nkx2.2 Ventral Midline Ventral Midline = Floorplate Conserved Patterning of the Neuroectoderm? DrosophilaNeuroectoderm

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