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BIOL 204 Lab For Week 9

BIOL 204 Lab For Week 9. Respiratory System Physiology and Histology. Objective 1 Trachea - Histology. Identify the pseudostratified ciliated epithelium, the hyaline cartilage and smooth muscle (if visible). Tracheal Epithelium. A lower magnification – tracheal cartilage.

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BIOL 204 Lab For Week 9

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  1. BIOL 204 Lab For Week 9 Respiratory System Physiology and Histology

  2. Objective 1 Trachea - Histology Identify the pseudostratified ciliated epithelium, the hyaline cartilage and smooth muscle (if visible)

  3. Tracheal Epithelium

  4. A lower magnification – tracheal cartilage

  5. Objective 2 Lung Tissue Identify bronchioles, alveolar sacs, alveolar ducts, bronchi and alveoli

  6. Can You Tell The Difference?????

  7. Objective 3 Pressure/Volume Changes During Ventilation

  8. If you pretend that the balloons are lungs, and that the latex sheet on the bottom is the diaphragm…….. What happens when you What happens when you push the diaphragm up? pull the diaphragm down?

  9. Objective 4, 5 and 6Spirometry Can be used to measure lung volumes and calculate lung capacities

  10. A Hand Held Spirometer is used to measure 1. tidal volume (TV) 2. expiratory reserve volume (ERV) 3. vital capacity (VC)

  11. Use these values to calculate: Minute Respiratory Volume (MRV) = Tidal Volume X respirations/Minute Inspiratory Reserve Volume (IRV) = VC – (TV + ERV) Inspiratory Capacity (IC) = TV + IRV

  12. Predicted Vital Capacity Males = 0.052 (height) – 0.022 (age) – 3.60 Females = 0.041 (height) - 0.018 (age) - 2.69 VC = vital capacity in liters H = height in centimeters A = age in years average measured VC % of predicted vital capacity = X 100 predicted value (from tables)

  13. Objective 7 Acid Base Balance Plasma buffers exist to maintain plasma pH between 7.35 and 7.45 NaHCO3 (sodium bicarbonate) buffers H+ and prevents acid fluctuations: NaHCO3+ H+ HHCO3+ Na+(sodium bicarbonate) H2CO3(carbonic acid) buffers OH- and prevents alkaline fluctuations: H2CO3+ OH-HCO3- + H+OH- (carbonic acid)

  14. The respiratory system controls plasma levels of CO2 and thus controls plasma levels of H2CO3: Hypoventilationleads to increased H2CO3 and decreased pH and Hyperventilationleads to decreased H2CO3 and increased pH

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