1 / 8

Chapter 25

Chapter 25. The RNA World. microRNA. Previously thought to be “junk” DNA Now determined to “code” for other RNA ENCODE project Andrew Fire and Craig Mello Nobel Prize in 2006 for miRNA Epigenetic control. miRNA pathway. Pol II makes RNA transcript

kioshi
Download Presentation

Chapter 25

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Chapter 25 The RNA World

  2. microRNA • Previously thought to be “junk” DNA • Now determined to “code” for other RNA • ENCODE project • Andrew Fire and Craig Mello • Nobel Prize in 2006 for miRNA • Epigenetic control

  3. miRNA pathway • Pol II makes RNA transcript • Drosha and other proteins trim the long pri-miRNA • Exportin 5 takes pre-miRNA out of nucleus • Dicer cleaves loop and separates strands? • RISC carries guide strand and releases passenger strand (degraded) • Basepairing with mRNA causes • Silencing if perfect pairing • Knockdown if imperfect (3’ UTR)

  4. Other RNAi Mechanisms (compare)

  5. shRNA or siRNA • This is the same as miRNA, but man-made • Must be designed carefully • Must be less than 30 nt • Otherwise degraded by host imune system • Cannot have strong hairpin loop • Want complementary strand to bind to RISC • Need a viral vector (manufacturer of the RNA)

  6. Health and Disease • Can currently silence genes in cell culture • How to use them in organisms? • Targets, specificity? • Learning tools in animal models

  7. Final Thought • Is DNA in control, or is RNA in control? • RNA world hypothesis may never have gone away

More Related