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Artemia franciscana

Artemia franciscana. North American brine shrimp. (Crustacea, Anostraca). fertilized eggs in brood sac. Artemia life cycle. winter ‘eggs’. Adult female. HATCH. Artemia franciscana (dry, encysted ‘winter eggs’). ‘ Paint’ dry cysts on double-stick tape with small, soft, dry paintbrush.

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Artemia franciscana

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  1. Artemia franciscana North American brine shrimp (Crustacea, Anostraca)

  2. fertilized eggs in brood sac Artemialife cycle winter ‘eggs’ Adult female HATCH

  3. Artemia franciscana(dry, encysted ‘winter eggs’)

  4. ‘Paint’ dry cysts on double-stick tape with small, soft, dry paintbrush

  5. Method Apaint cysts on sticky circle

  6. Method B plastic petri dish transparency strip with grid10 mm x 10 mm strip of double-stick tape covers grid tape holds strip to dish

  7.  Cysts painted on sticky grid with brush

  8. STUCK-onARTEMIATechnical Advantages easy handling & transfer of cysts uses small, quantifiable number of cysts follow development of individual cysts cysts stay in single focal plane use dissecting or compound microscope manipulation of many relevant variables

  9. A1 Hatching of encystedArtemia embryos A1-A2: Encysted late gastrula stage(immersed in sea water and stuck to tape)B: Cyst hydrates and swells; cyst wall splitsC1-C2: “Umbrella” stage emerges (membrane-bound)D1-D2: Nauplius larvae are free swimming C1 A2 C2 B D1 D2

  10. Each day, use pipet to remove and count hatched, swimming nauplius larvae 

  11. CONTROL 50 0 0 0% 22 22 44% 66% 11 33 6 39 78% 1 40 80%

  12. Typical hatching curve: %HATCH

  13. Examples of experimental variables that may affect hatching curves 1. dry cysts exposed to thermal extremes 2. ambient light effects during hydration 3. temperature effects during hydration 4. salinity effects 5. second hydration 6. oxygenation effects during hydration 7. environmental toxicant effects 8. irradiation effects on dry or hydrating cysts

  14. Measurements & End-points * Hatching % *hatching time-coursedevelopmental anomalieslarval motility molting & growth

  15. 'N Cyst A sea monkey's no monkey or chimp. It's crustacean - better known as brine shrimp.This genus Artemia Can deal with extremia.As a cyst stage, its hardly a wimp.

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