NSLS-II Life Science Breakout Session

1. NSLS-IILife Science Breakout Session

2. Agenda • Introduction (Miller) • Keynote Speaker: Carolyn Larabell (ALS, UCSF) (30 min) • Technique talks (4 min each) • Imaging • Soft x-ray spectromicroscopy (Jacobsen) • Diffraction Imaging (Jacobsen) • Full-field transmission x-ray microscopy (Larabell) • X-ray fluorescence micro/nanoprobe (Vogt) • Diffraction-enhanced imaging (Zhong) • Microbeam radiation therapy (Dilmanian) • Infrared microspectroscopy and imaging (Miller) • Structural & Molecular Biology: • X-ray footprinting (Chance) • XANES/EXAFS (Chance) • Macromolecular Crystallography (Stojanoff) • Small-Angle X-Ray Scattering (Yang) • Discussion • Closeout (Sweet)

3. Life Sciences Discussion Session Summary • What are the key scientific drivers? What experiments will NSLS-II enable that are not presently possible? • What technical capabilities will these require? (Beamlines, endstations, undulators…) • Estimate of community size. • What detector requirements does this field have? Do these require R+D? • What software and computing infrastructure requirements are there? (Control, data acquisition, analysis) • Any particular accelerator requirements? • Any particular conventional facility requirements?

4. Key Scientific Drivers Cross-Technique Imaging • In identical biological cells: • infrared imaging: chemical imaging of biological cells • full-field, soft x-ray TXM: nanoscale 3D sub-structure • x-ray spectromicroscopy: 3D nanoscale chemical imaging of organic components • x-ray fluorescence microprobe: 3D trace element mapping Cross-Technique Structural/Molecular Biology • For the same protein: • small-angle x-ray scattering: low-resolution structure, static & dynamic • EXAFS: metal active site environment, static & dynamic • MX: atomic-resolution structure, static & dynamic Life Sciences “Village” Environment

5. Technical Capabilities • Imaging • Soft x-ray spectromicroscopy (ID for tomography) • Diffraction Imaging (ID) • Full-field transmission x-ray microscopy (BM) • X-ray fluorescence micro/nanoprobe (ID) • Diffraction-enhanced imaging (SCW) • Microbeam radiation therapy (ID) • Infrared microspectroscopy and imaging (2 BM) • Structural & Molecular Biology: • X-ray footprinting (3PW) • XANES/EXAFS (DW) • Macromolecular Crystallography (6 IDs) • Small-Angle X-Ray Scattering (1 ID, 1 3PW)

6. Community Size 3000 2500 2000 # Users 1500 45% in 2006 1000 500 0 1990 1991 1992 1993 1994 1995 1996 1997 1998 1999 2000 2001 2002 2003 2004 2005 2006 Fiscal Year Chemical Sciences Materials Sciences Life Sciences Environmental and Geoscience Applied Science and Engineering Optical/Nuclear/General Physics Unspecified

7. 1000 900 800 700 600 500 400 300 200 100 0 2002 2003 2004 2005 2006 Community Size TOTAL Life Sciences • 45% of NSLS publications are by life sciences users* • 65% macromolecular crystallography • 30% other structural/molecular biology (spectroscopy, SAXS, footprinting) • 5% imaging *FY06

8. Life Sciences “Village” Environment • Accelerator • Meet specs • Detectors • Limiting today for: MX, EXAFS, IR • Laboratories and ancillary facilities • Wet lab, with basic equipment (e.g., BSL2, cell culture, crystal growth, protein purif.) • Light, epifluorescence, confocal microscopes • Sample prep, in particular freezing, microtoming (ultra, cryo, RT) • Access to correlated (same mounts ?) electron, IR, and soft x-ray microscopies • Adequate staffing !!!!!! • Including people with significant background in biological sample prep. • Only supporting beamline ops is insufficient, need to support whole experiment (planning, sample prep, data analysis, interpretation)! • IT • Handling of very large datasets • ‘full’ remote control of beamline, remote access; cybersecurity, firewall issues • Quality of Life • Offices for beamline staff NEAR beamline; lab space near beamline • Sufficient floor space at beamline, AND office like controls environment