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About OMICS Group

About OMICS Group.

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About OMICS Group

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  1. About OMICS Group OMICS Group International is an amalgamation of Open Access publications and worldwide international science conferences and events. Established in the year 2007 with the sole aim of making the information on Sciences and technology ‘Open Access’, OMICS Group publishes 400 online open access scholarly journals in all aspects of Science, Engineering, Management and Technology journals. OMICS Group has been instrumental in taking the knowledge on Science & technology to the doorsteps of ordinary men and women. Research Scholars, Students, Libraries, Educational Institutions, Research centers and the industry are main stakeholders that benefitted greatly from this knowledge dissemination. OMICS Group also organizes 300 International conferences annually across the globe, where knowledge transfer takes place through debates, round table discussions, poster presentations, workshops, symposia and exhibitions.

  2. About OMICS Group Conferences OMICS Group International is a pioneer and leading science event organizer, which publishes around 400 open access journals and conducts over 300 Medical, Clinical, Engineering, Life Sciences, Phrama scientific conferences all over the globe annually with the support of more than 1000 scientific associations and 30,000 editorial board members and 3.5 million followers to its credit. OMICS Group has organized 500 conferences, workshops and national symposiums across the major cities including San Francisco, Las Vegas, San Antonio, Omaha, Orlando, Raleigh, Santa Clara, Chicago, Philadelphia, Baltimore, United Kingdom, Valencia, Dubai, Beijing, Hyderabad, Bengaluru and Mumbai.

  3. Track 9, 15:00-15:20 Aug., 26, 2014 Pharmacognosy 2014, Beijing Neurotrophic Compounds of Javanese Ginger, Zingiber purpureum Yoshiyasu Fukuyama Pharmaceutical Sciences, Tokushima Bunri University Tokushima, Japan

  4. Drugs for Treatment of Alzheimer’s Disease

  5. Effects of Neurotrophins Neurotrophins (NGF, BDNF, NT-3, NT-4/5 ) Neuronal stem cells Differentiation Neuronal precursor cells Development Maturation Ageing Death Astrocyte Neurons Oligodendrocyte

  6. Neurotrophic factors • Representative neurotrophic factors • ・NGF • ・ BDNF • ・ NT-3, NT-4/5 • Problems due to large peptide molecule • ・No penetration of B.B.B. • ・Poor oral pharmacokinetic profiles • ・Immune reactions Nonpeptidyl small molecule neurotrophic compounds

  7. PC12 cells used for Screening neurotrophic/neuroprotective/cytotoxic compounds “The PC12 cell line was derived from rat pheochromocytoma, a tumor arising from chromaffin cells of the adrenal medulla. It is a useful model for studying cell signaling for at least two reasons: (i) There are few growth factors, neurotrophins, and hormones to which it does not respond; and (ii) distinct responses of differentiation, proliferation, and survival can all be assessed independently.” Neuroprotectiveafter NGF withdrawal Science, 296(5573):1648 (2002) Neurotrophic Cytotoxic

  8. The Principles for Screening Neurotrophic/Neuroprotective Compounds in Rat Cortical Neurons Neurotrophic compounds can promote neurite outgrowth, which improves the status of neurons. Neuroprotective compounds can retard toxic factor-induced cell death in primary cultured neurons. Neurotrophic toxic

  9. Search for Neurotrophic Mimic Natural Products Rat pheochromocytoma cell line (PC12 cells) Primary cultured rat neurons (Cortical neurons) Neurotrophic Compounds Library

  10. BANGLE (Zingiber purpureum) This plant is used as a spice and also used for traditional Indonesian medicine ‘jamu’. Purpose: Rhizome: Fever, headache Cough, expectorate Stomach ache Constipation Jaundice Rheumatism The ingredients of herbal medicine for women after childbirth. Leaf: Revitalizer  Digestive trouble

  11. BANGLE (Zingiber purpureum) NGF-like Activity of BANGLE in PC12 Cells PC12 Cells MeOH ext (25 μg/mL) Absence of NGF Ctrl ( 0.5% EtOH )

  12. : Neurotrophic activity (+) Ctrl ( 0.5% EtOH ) Fr.4 ( 12.5 μg/mL ) Fr.5 ( 12.5 μg/mL )

  13. Screening Result of Compounds from BANGLE by PC12 Cells

  14. Differentiation of PC12 Cells Induced by 1 and 2 Control (0.5 % EtOH) NGF (10ng/mL) 1 (30 μM) 2 (30 μM) PC12 cells were cultured in 24-well plates in DMEM + 10% HS and 5% FBS for 24 h at a density of2x103 cells/cm2, and then medium was changed to DMEM + 2% HS and 1% FBS containing1 and 2. After 4 days cells with neurites were counted. ## P<0.01 vs control; by Chi-square test

  15. Neurite Length of NGF-differentiated PC12 Cells Effected by 1 Average of neurite length (μm ) 1 PC12 cells were cultured in two 24-well plates in DMEM + 10% HS and 5% FBS for 24 h at a density of 2 x103 cells/cm2, and then medium was changed to DMEM + 2% HS and 1% FBS with NGF (10 ng/mL) containing 19 respectively. After 4 days neurite lengths of PC12 cells were quantified. Data are expressed as the mean ± SE (n= 150). **P < 0.01 vs control; Dunnett’s t test.

  16. Protocol for screening Neurite outgrowth- promoting activity Change medium in the absence or presence of sample compounds Seeding 6 day 1 day Fixed by PFA DMEM/10 % FBS NB/2 % B27 overnight Neuron density : 5 x103 cells/cm2 Stained with anti-MAP2

  17. Neurite Outgrowth of Cultured Rat Cortical Neurons Promoted by 1 and 2 control 1 (3 μmol/L) bFGF (10 ng/mL) 2 (3 μmol/L) Neuronal cells were first cultured in 24-well plate in DMEM/10% FBS for 1 day at the density of 5000 cells/cm2, and then medium was changed to serum-free Neurobasal medium plus 2% B27 supplement with or without 1, 2, (+)-1, (-)-1, and bFGF (10 ng/mL). After 6-days treatment of 1 and 2 neurons were fixed and stained with anti-MAP2 immunohistochemical method.

  18. Quantitative Analysis of the Neurite Outgrowth of 1, 2, (+)-1, and (-)-1 1 (μM) 2 (μM) (+)-1 (μM) (-)-1 (μM) After the neuronal cells (5000 cell cm-2) cultured for 7d in the presence of 0.5% EtOH, bFGF, 1, 2, (+)-1, and (-)-1 were fixedwith 4% paraformaldehyde, morphometric analysis was carried out on these neurons according to the criteria described. The data are expressed ±S.E. (n=140); Dunnett's t-test vs. control, **p<0.01.

  19. Neurite Number from Cell Body 2 (μM) 1 (μM) After the neuronal cells (5000 cell cm-2) cultured for 7d in the presence of 0.5% EtOH, bFGF, 1, and 2 were fixedwith 4% paraformaldehyde, morphometric analysis was carried out on these neurons according to the criteria described.The data are expressed ±S.E. (n=60); Dunnett's t-test vs. control, **p<0.01.

  20. Synthesis of Phenylbutenoid Dimers 1. Pittaya Tuntiwachwuttikul, Brin Limchawfar, Vichai Reutrakul, Orasa Pancharoen, Kosan Kusamran Lindsay T. Byrne, Aust. J. Chem.1980, 33, 913-916.

  21. Neurogenesis of Phenylbutenoid Dimers in OBX Mice memory impairment deppression Olfactory Bulbectomized (OBX )Mice neurodegenerative model mice olfactory bulb OBX Animals ddY (8W) Schedule 0 7 14 21 28 day SSRI : Selective Serotonin Reuptake Inhibitors OBX BrdU (50mg/kg I.p.) IHC (BrdU / NeuN) Comp.1 (50mg/kg/day p.o.) Comp.2 (50mg/kg/day p.o.) fluoxetine (10mg/kg/day i.p.) IHC : Immunohistochemistry NeuN: Neuron specific nuclear protein BrdU: 5-Bromo-2’-deoxyuridine

  22. Protocols Brain hippocampal immunohistochemistry Confocal laser-scanning microscope Double-labeled for NeuN and BrdU BrdU(green) NeuN(red) Merged (yellow) 100μm 100μm 100μm

  23. Confocal microscopy images of double staining for BrdU and NeuN in DG regions of the hippocampus. non sham OBX OBX Veh. (i.p.) OBX FLU. (i.p.) OBX Veh. (p.o.) OBX 1 (p.o.) OBX 2 (p.o.) BrdU NeuN Merged

  24. Quantitative analysis of the number of BrdU and NeuN coexpressing cells in DG regions of the hippocampus Comp.1 (50mg/kg/day p.o.) Comp.2 (50mg/kg/day p.o.) fluoxetine (10mg/kg/day i.p.) non: non-operated age-mached mouse sham: operated mouse with olfactory bulb veh: 0.5% CMC

  25. 1H NMR spectrum (600 MHz, C6D6) of 3 Cassumunarin A (7)1) [α]D = + 71.3 (c 0.1, MeOH) IR ν max : 3389 (OH), 1658 (C=O), 1579 (arom.) cm-1 UV λ max (ε) : 205 (44360) nm FAB-MS : m/z 609[M+K]+ HR-FAB-MS : found 609.1819 calcd 609.1819 for C34H34O8K 28 ATR MeOH 1) Tom J. Mabry, Tetrahedron Letters,35, No. 7, pp. 981-984, 1994

  26. Cassumunarin A δC = 43.6 ppm HMBC of 3

  27. Plausible biosynthesis of compound 3

  28. Neurite Outgrowth of PC12 Cells Promoted by 3 Ctrl (0.5% EtOH) NGF (2 ng/mL) 1 μM + NGF (2 ng/mL) 10 μM + NGF (2 ng/mL)

  29. Neurons Dead neurons Neuronal stem cells Neurodifferentiation Neuroprotective activity Neurite outgrwth-promoting activity Neurotrophic Compounds from BANGLE

  30. Lets Meet again at Pharmacognosy-2015 3rd International Conference and Exhibition on Pharmacognosy, Phytochemistry and Natural Products October 26-28, 2015 Hyderabad, India Theme: Advanced trends for the future of Herbal Drugs and Products Website:http://pharmacognosy-phytochemistry-natural-products.pharmaceuticalconferences.com/

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