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Lecture 7

Lecture 7. August 15, 2005 Lehninger (4 th Edition), Chapter 16 Oxidative phosphorylation: Chapter 19. Products from one turn of the Krebs cycle. LNC Fig.16.13. the only membrane- bound enzyme in the TCA cycle. LNC Fig.16.7. THE GLYOXYLATE CYCLE. LNC Chapter 16, pages 623-626.

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Lecture 7

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  1. Lecture 7 • August 15, 2005 • Lehninger (4th Edition), Chapter 16 • Oxidative phosphorylation: Chapter 19

  2. Products from one turn of the Krebs cycle LNC Fig.16.13

  3. the only membrane- bound enzyme in the TCA cycle LNC Fig.16.7

  4. THE GLYOXYLATE CYCLE LNC Chapter 16, pages 623-626

  5. from oxidation of fatty acids C2 C4 C6 C4 C6 C4 C2 C2 LNC Fig.16-20 Note that the figure 16-16 in the 3rd edition has an error (there is an O instead of an H)

  6. LNC Fig.16.22

  7. isocitrate lyase: inactivated by low ATP (high AMP/ADP) isocitrate dehydrogenase: inactivated by phosphorylation LNC Fig.16.23

  8. ELECTRON TRANSPORT AND OXIDATIVE PHOSPHORYLATION Chapter 19 in LNC

  9. CO2 HEAT HEAT CO2 “Guinea Pig” “Charcoal”

  10. “…..this fire stolen from heaven, this torch of Prometheus, does not only represent an ingenious and poetic idea, it is a faithful picture of the operations of nature, at least for animals that breathe... the torch of life lights itself at the moment the infant breathes for the first time, and it does not extinguish itself except at death.” Lavoisier, 1789

  11. Carbohydrate + oxygen => carbon dioxide and water C6H12O6 + 6 O2 => 12 H2O + 6 CO2 + ENERGY

  12. RESPIRATION

  13. 2

  14. Wild type SDHC

  15. Outer membrane Inner membrane Matrix Intermembrane space

  16. LNC Fig.19.7

  17. the arrangement of the complexes in the inner membrane and the order of electron flow

  18. NADH

  19. rotenone TMPD/ascorbate antimycin malonate Glutamate/malate CN- succinate Succinate dehydrogenase membrane bound enzyme of Krebs cycle Four integral membrane protein complexes Two mobile carriers: ubiquinone and cytochrome c LNC Fig.19.15

  20. LNC Fig.19.8

  21. lipid-soluble polyisoprene chain oxidized Coenzyme Q or Q reduced Coenzyme Q or QH2 LNC Fig.19.2

  22. Heme + apoprotein cytochrome LNC Fig.19.3

  23. Structure of cytochrome c

  24. LNC Fig.19.4

  25. NON-HEME IRON - SULFUR CENTERS [Fe2-S2] [Fe4 - S4]

  26. LNC Fig.19.5

  27. [Fe2-S2] LNC Fig.19.5

  28. [Fe4-S4] LNC Fig.19.5

  29. A bacterial ferredoxin LNC Fig.19.5

  30. O2 NADH

  31. 7 or 8 NADH + Q + H+ NAD+ + QH2 LNC Fig.19.9

  32. Architecture of Succinate Dehydrogenase and Reactive Oxygen Species Generation Victoria Yankovskaya,1* Rob Horsefield,2* Susanna To¨rnroth,3* Ce´sar Luna-Chavez,1,4† Hideto Miyoshi,5 Christophe Le´ger,6‡ Bernadette Byrne,2 Gary Cecchini,1,4§ So Iwata2,3,7§ SCIENCE 31 JANUARY 2003 VOL 299, p.700 www.sciencemag.org succinate [2Fe-2S] [3Fe-4S] [4Fe-4S] Succinate + Q  fumarate + QH2 LNC Fig.19.10

  33. Complex III 8-11 polypeptides two cytochromes, b and c1 one iron-sulfur center QH2 + 2 cyt c (Fe+3)  Q + 2 cyt c (Fe+2) (ignore the protons for the moment) LNC Fig.19-11

  34. Complex III 8-11 polypeptides two cytochromes, b and c1 one iron-sulfur center LNC Fig.19-11b

  35. The Q Cycle LNC Fig.19-12 Net equation QH2 + 2 cyt cox + 2H+in Q + 2 cyt cred + 4H+out 2 Fe+3 2 Fe+2

  36. Cyt c1red + Cyt c ox Cyt c1ox + Cyt c red Fe+2 Fe+3  Fe+3 Fe+2

  37. Complex IV - cytochrome oxidase 9 - 13 polypeptides cytochromes a and a3 two copper centers 4 cyt c (Fe+2) + O2 + 4H+  4 cyt c (Fe+3) + 2 H2O ( and protons pumped) LNC Fig19-13a

  38. Complex IV (schematic) 4 cyt cred + O2 + 4 H+ 4 cyt cox + 2 H2O Fe+2 Fe+3 LNC Fig.19-14

  39. NADH + ½ O2 + H+ NAD+ + H2O DE’o = +0.816 v + 0.320 v = + 1.136 v DG’o = -n FDE’o = - 2 x 96,480 J/Vxmole x 1.136 v = - 220 kJ/mole NADH An excerpt from Table 14-7, in reverse order

  40. End of Lecture 7 Mon, Aug. 15, 2005

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