1 / 6

Video Demo of Some Key Steps of AimPlex Assays

Video Demo of Some Key Steps of AimPlex Assays. Please refer to the AimPlex assay user manual for details of the assay procedure. Reconstitution of Lyophilized Standard(s). Quick spin the antigen vial before adding standard diluent . Vortex gently for 15 sec.

kishi
Download Presentation

Video Demo of Some Key Steps of AimPlex Assays

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Video Demo of Some Key Steps of AimPlexAssays Please refer to the AimPlex assay user manual for details of the assay procedure

  2. Reconstitution of Lyophilized Standard(s) • Quick spin the antigen vial before adding standard diluent. • Vortex gently for 15 sec. • Incubate on ice for 5-10 min

  3. Serial Dilution Preparation • Transfer the indicated volume from the previous concentration • Mix each addition by pipetting up and down 6–8 times. • Change pipette tips after each addition to avoid contamination from one concentration to another.

  4. Seal Empty Wells with a Plate Sealer • Mark the wells. • Seal unused wells with a plate sealer • Those wells can be used in future experiment(s).

  5. Plate Wash using the AimPlex Filter Plate Washer • Adjust vacuum pressure so that it takes 3-5 seconds to clear 100 μL 1x Wash Buffer from the wells • Gently tap the plate bottom onto several layers of paper towels to remove residual buffer on the plate bottom after the wash.

  6. Incubation • Cover the plate with a plate seal • The filter plate should be placed on top of the filter plate lid • Wrap the plate (on top of the plate lid) with aluminum foil to protect from light • Incubate on the shaker (set at 700 rpm) at room temperature.

More Related