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Thermal cycler or a DNA amplification machine performs polymerase chain reactions to make copies of genetic material and is popularly known as a PCR. The machine raises and lowers temperature at a highly sensitive pre-programmed steps in order to denature and renature DNA that is inserted within small tubes of the machine. The amplification of DNA is a precursor in experimentation and research in genomics and proteomics to study drug interactions, genome sequencing, protein sequencing and gene identification. PCR was introduced a few decades ago and have undergone revolutionary changes in terms of quality and speed. Although the working mechanism for a PCR is simple, their cost is primarily high due to the presence of highly sensitive temperature control system. Peltier and silver elements are primarily used in thermal cyclers to achieve temperature control in a PCR instrument. The term instant PCR signifies instant evaluation of the absolute or relative quantification of the cDNA copies from a single real time PCR curve. Instant PCR enables amplification of DNA strand in a short time period for formation of a number of identical DNA copies.
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