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"Mechanisms and sources of resistance"

Expert Group 2. "Mechanisms and sources of resistance". Boulton lab (JIC):. Mastrevirus genus of the Geminiviridae (mainly infect cereals e.g. Wheat dwarf virus (WDV) and Maize streak virus (MSV)). WDV : Europe (Scandinavia → France →Czech Rep., Hungary).

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"Mechanisms and sources of resistance"

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  1. Expert Group 2 "Mechanisms and sources of resistance" Boulton lab (JIC): Mastrevirus genus of the Geminiviridae (mainly infect cereals e.g. Wheat dwarf virus (WDV) and Maize streak virus (MSV)) WDV: Europe (Scandinavia →France →Czech Rep., Hungary) MSV: Sub-Saharan Africa and Indian Ocean islands Interests: Mastrevirus-host interactions (susceptible and resistant) • Interaction of virus early gene products with host proteins • (modulation of host cell cycle, virus replication) • characterisation of resistance in cereals

  2. Interaction of geminivirus early gene products with host proteins Bean yellow dwarf virus and Beet curly top virus + Arabidopsis Identification of pathways involved (e.g. microarray analysis of transgenic plants inducibly expressing Rep, RepA and C4) Identification of susceptibility factors – candidate genes and genes identified in suppressor screens following ems mutagenesis Basis for geminivirus resistance via TILLING populations

  3. H Inf Biological and genetic characterisation of resistance to geminiviruses (WDV) in cereals Biological characterisation: Identification of resistant/tolerant cultivars: a) Vector transmission: Psammotettix alienus + WDV-[CZ] (wheat) and WDV-[HU] (barley) Established colony and conditions JIC insectary, with Ian Bedford) WDV-[HU] in barley Psammotettix alienus Insect collection

  4. Biological and genetic characterisation of resistance to geminiviruses (WDV) in cereals Biological characterisation: b) Agroinoculation: Mechanism for inoculation of cloned DNA Established conditions for inoculation of seedlings at different developmental stages (barley and wheat) Seed inoculation (barley) Seedling inoculation (wheat) WDV-[CZ] in wheat Two techniques: Differentiate between resistance to virus replication/insect transmission

  5. Triumph Igri Biological and genetic characterisation of resistance to geminiviruses in cereals Genetic characterisation: (of HvMsv1, with Dave Laurie) Map based cloning: Using doubled haploid and F2, F3 populations barley cvs: Igri x Triumph Transcript based cloning: Bulked segregant approach Candidate genes

  6. Potential for ResistVir (JIC Boulton group) [WDV, wheat and barley] • Methods for screening susceptibility of germplasm: cloned and natural isolates (agroinoculation and vector transmission) 2. Mapping technologies for identification genes/markers: wheat and barley) Improved resistance to WDV in cereals

  7. Collaborative potential/synergy/overlap? WDV: Partner 22 (Aschersleben, Germany) Partner 26 (NIAB –Kenyon) Susceptibility factors: Partner 39 • Assist further progress? • Information: Availability of germplasm: RILs, doubled haploids, mutant populations • Information/access: Tilling populations produced • Access to high throughput marker technology

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