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Do Now

Do Now. Walk Around The Room And Look At the Flip Books We Made. 1) Choose 5 Books To Rate 2) Please Rank the Books from 1- 3 based on: Creativity – 1 point Appeal -1 point Explanation of Enzymes-1 point Please Mark On Board your choices. Class Day. Discuss Enzyme Books

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Do Now

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  1. Do Now Walk Around The Room And Look At the Flip Books We Made. 1) Choose 5 Books To Rate 2) Please Rank the Books from 1- 3 based on: Creativity – 1 point Appeal -1 point Explanation of Enzymes-1 point Please Mark On Board your choices

  2. Class Day • Discuss Enzyme Books • Write Procedure for Wednesday’s Lab • Homework – Re-read Chapter 2 to prepare for test. Answer questions 1-10 on page 57 and 1-10 on page 59 to prepare for test.

  3. Liver Enzyme Catalase Lab • H2O2  H2O + O2 • Hydrogen Peroxide when exposed to liver enzyme catalase produces water and oxygen.

  4. Liver Enzyme Lab • Please Prepare to Write Down The procedure for Wednesday’s Lab • Title • Effects of Temperature on Enzyme Activity

  5. Materials • Raw liver • Petri dish • Dropper pipette • 1% hydrogen peroxide • 25 ml graduate cylinder • 3 beakers • Filter paper disks • Forceps • Ice • Heating plate • Clock • Paper towel

  6. Hypothesis • Please formulate your own hypothesis regarding the rate of oxygen production and the temperature of the liver • Write down your prediction NOW

  7. Procedure • Put on safety gloves and goggles. • Use forceps to dip a piece of filter paper into the room temperature liver. • Place the filter-paper disk on a paper towel to remove any extra liquid. • Use forceps to place the filter-paper disk at the at the bottom of the hydrogen peroxide solution

  8. Procedure • Observe the filter paper and record what you see AND the amount of time it takes for the filter paper to float to the top of the solution. • Using the same procedure as above repeat experiment for the liver that is heated and the liver that is on ice. • Record what happens in the reaction, liver temperature and time it takes for the filter paper to float.

  9. Let’s Go Beyond!!! • If you finish the lab early – please think of extension activities • Example : effects of pH – add lemon juice, record the time it takes for filter paper to float. What about the microwave???

  10. Questions to think About • What is the relationship between the catalase soaked paper and the amount os catalase activity in the sloution • How did the temperature affect catalase activity? • What was different about the potato reaction?

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