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1. IZLAGAČ dr Miroslav Demajo NSV Laboratorija za molekularnu biologiju i endokrinologiju

1. IZLAGAČ dr Miroslav Demajo NSV Laboratorija za molekularnu biologiju i endokrinologiju. 2. NAZIV OPREME Victor Wallac 1420 Multilabel Counter, Perkin Elmer. 3. GODINA NABAVKE 2003 godina. Pušten u rad 03.11.2003 god. 4. POREKLO SREDSTAVA

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1. IZLAGAČ dr Miroslav Demajo NSV Laboratorija za molekularnu biologiju i endokrinologiju

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  1. 1. IZLAGAČ dr Miroslav Demajo NSV Laboratorija za molekularnu biologiju i endokrinologiju

  2. 2. NAZIV OPREME Victor Wallac 1420 Multilabel Counter, Perkin Elmer

  3. 3. GODINA NABAVKE 2003 godina. Pušten u rad 03.11.2003 god.

  4. 4. POREKLO SREDSTAVA Ministarstvo za nauku i životnu sredinu Republike Srbije

  5. 5. OSNOVNE KARAKTERISTIKE Osposobljen za kvantitativnu detekciju svetlosno emitujuće i svetlosno absorbujuće markere. Luminometrija, fluorometrija, "flash" absorbanca, fotometrija. Ugrađen šejker, kontrola temperature (do 45°C). Uzorci se stavljaju na ELISA ploče sa bunarčićima, petri šolje.

  6. 6. RADNO (UPOTREBNO STANJE) Stalno spreman za upotrebu. Nije potrebna posebna pripremanakon uključivanja u struju.

  7. 7. LOKACIJA, ZADUŽENJE Laboratorija za molekularnu biologiju i endokrinologiju 090, soba 19. Zadužen dr Miroslav Demajo, tel-lokal 763

  8. 9. ISKORIŠĆENOST Od dana puštanja u rad i nakon obuke od strane servisera LKB, u stalnoj dnevnoj upotrebi. Vodi se knjiga upotrebe koja je dostupna na uvid. Pomoću instrumenta su u pripremi 6 doktorata i 1 magistratura. Za sada se vrše sledeće analize: Test proliferacija ćelija (Brdu-brom deoksid uridin). Test vijabilnosti ćelija (MTT-tetrazidin so). Test vijabilnosti i citotoksičnost ćelija (Sr-b, sulforodamin b). Hormoni u cirkulaciji.

  9. 10. KORISNICI Laboratorija za molekularnu biologiju i endokrinologiju "Vinča" Laboratorija za radioizotope "Vinča" IHTM-centar za hemiju, prethodni biotehnološki projekat 544. Instrument se koristi za realizaciju međunarodne saradnje sa Istituto Nazionale di Fisica, LNS, Catanija, Italija.

  10. 11. REZULATATI • A. Ristić-Fira, I Petrović, D. Todorović, L. Valastro, P. Cirrone, G. Cuttone, (2003), • Survival of HTB140 melanoma cells after exposure to 62 MeV protons. • INFN-LNS Activity Report 2003. • 2. A. Ristić-Fira, I Petrović, D. Todorović, L. Valastro, P. Cirrone, G. Cuttone, (2003), • Cell cycle phase redistribution and apoptosis of HTB140 melanoma cells • exposed to 62 MeV protons. INFN-LNS Activity Report 2003. • 3. I Petrović, D. Todorović, L. Valastro, P. Cirrone, G. Cuttone, A. Ristić-Fira, (2004), • Radiobiologicel studies on the 62 MeV therapeutic proton beam at LNS • Catania: I. Survival of HTB140 melanoma cells. Physical chemistry 2004, • Proceedings, 426-428. • 4. A. Ristić-Fira, D. Todorović, L. Valastro, P. Cirrone, G. Cuttone, I Petrović, (2004), • Radiobiologicel studies on the 62 MeV therapeutic proton beam at LNS • Catania: II. FACS analyses of HTB140 melanoma cells. Physical chemistry 2004, • Proceedings, 429-431. • 5. L. Korićanac, D. Todorović, N. Popović, A. Ristić-Fira, (2004), • Changes of c-myc expression in B16 melanoma cells • by 8-chloradenosine 3´,5´-monophosphat. Physical chemistry 2004, Proceedings, 413-415. • 6. I. Petrović, A. Ristić-Fira, D. Todorović, L. Valastro, P. Cirrone, G. Cutone, (2004), • Relative biological effectiveness of high energy protons for a human melanoma, • YUNSC, Proceedings, 161-164.

  11. 7. A. Ristić-Fira, I Petrović, D. Todorović, L. Valastro, P. Cirrone, G. Cuttone, Radiobiological analysis of human melanoma cells on the 62 MeV CATANA proton beam. submitted to: International Journal of Radiation Biology, (under review after author correction 2005). 8. V.Vučić, M.Ađić, S. Ruždijić*, M.B.Radojčić: Comparison of gamma radiation-induced effects in two human prostate cancer cells. Proceedings YUNSC 2004. 9. Vučić, V., Adžić, M., Ruždijić,S., and M. B. Radojčić: Comparison of Gamma Radiation-Induced Effects in two Human Prostate Cancer Cell Lines5thInternational Yugoslav Nuclear Society Conference, Belgrade, Serbia and Montenegro (2004) Book of Abstracts, No.F3.2., p.45 10. Vučić,V., Ruždijić S., and M.B.Radojčić: Cytotoxic and antiproliferative effects of Ionizing Radiation and Nucleosise analogs on DU-145 Human Prostate Cancer Cells29th FEBS Congress,26 June – 1 July 2004, Warsaw,POLAND, Book of abstracts, pp. 130, P4.1-06.11. Vučić V., Radojčić B.M., and Ruždijić S. Cell growth inhibition in PC-3 human prostate cancer cells after treatment with gamma-radiation and 8-Cl-cAMP29th ESMO Congress (European society for medical oncology), Vienna, Austria29. Oct - 2. Nov, 2004. No 431P12. Vučić,V., Janković,D., Medić-Miješević,Lj., Radojčić,M.B., i S.Ruždijić: Antitumorski efekti tiazofurina i 8-Cl-cAMP na ćelije humanog kancera prostate. Simpozijum: Nukleozidi, nukleotidi i njihovi analozi u biohemijskim istraživanjima, SANU, Beograd (2004) Zbornik sažetaka str 31-32.

  12. 12. MOGUĆNOST U pripremi analiza kinetike ATP u tkivu jajnika i uterusa sisara pod delovanjem teških metala i drugih faktora sredine bioluminiscencija; ATP+D-luciferin+PPi(2 fosfora)+AMP+CO2 +svetlo Šira mogućnost za rutinske analize u medicinskoj i veterinarskoj dijagnostici kao i analize za potrebe poljoprivrede.

  13. 13. POTREBE Instrument je kompletiran

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