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Your profession is your passion. Pass it on.

Your profession is your passion. Pass it on. bio-rad.com/scienceambassadors. Genes in a Bottle Activity. Today ’ s Activity. Focuses on isolating your own DNA from cheek cells The DNA will be transferred into an amulet

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Your profession is your passion. Pass it on.

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  1. Your profession is your passion. Pass it on. bio-rad.com/scienceambassadors

  2. Genes in a Bottle Activity Today’s Activity • Focuses on isolating your own DNA from cheek cells • The DNA will be transferred into an amulet • The amulet will be a permanent keepsake of your genetic material — your personal essence!

  3. Genes in a Bottle Activity Timeline Introduction Background on DNA Extraction Extract Genomic DNA from Cheek Cells Prepare DNA Necklaces

  4. What is DNA?

  5. If you pull out one of your hairs, what can you see?

  6. Hair cells are found in the root of the hair

  7. Hair cells have information about your hair

  8. A Cell What are the parts of an animal cell?

  9. Cells come in all shapes and sizes All have DNA!

  10. DNA is inside cells Cells are liken bags that hold DNA Break open the bags and out comes the DNA!

  11. Protocol Highlights Step 1: Use a simple water mouth wash to collect cheek cells Step 2: Add lysis buffer to cells to break open cell and nuclear membranes and release nuclear contents Step 3: Precipitate DNA with cold alcohol in high salt Step 4: Transfer your DNA into the amulet

  12. Illustrated Guide of Kit Components Plastic transfer pipet 15ml conical tube Micro test tube Amulet

  13. Workstation Inventory Zip-Top Bag Components • 15 ml conical tube 1 • Colored micro test tube with protease/salt/lysis buffer 1 • Disposable plastic transfer pipet 1 • Necklace components 1 • Tattoo 1 • Small paper cup 1

  14. Step 1: Cheek Cell Collection In this step, you will collect your cheek cells in a 15 ml conical tube using a water mouth wash. Take out the 15 ml conical tube which contains 3 ml of water and label with your initials. Practice using the transfer pipet by transferring 1 ml of water from a large cup to your small cup. Repeat 3 times for a total of 3 ml of water. Take the water from the small cup into your mouth — don’t swallow it! Swish the water around like mouthwash and gently chew on the inside of your cheeks while you swish the water around for 30–60 seconds. Carefully expel the water back into the small paper cup. Pour the water-cheek cell mix back into your 15 ml tube.

  15. Step 2: Cell Lysis and Proteolysis In this step, you will lyse your cheek cells and degrade the protein with a protease. Open the colored micro test tube containing a protease/salt/lysis buffer mixture. Pour the entire contents into the 15 ml conical tube containing your cheek cells. Place the cap on the tube and gently invert it 5 times — don’t shake it! Observe your tube — do you notice any changes? Incubate for 5 minutes by warming the tube in your hand. This allows time for the protease to break down the proteins.

  16. Step 3: DNA Precipitation In this step, you will precipitate your DNA with the addition of alcohol. Hold your tube at a 45°angle and slowly fill the tube with alcohol by gently pouring or pipetting alcohol down the inside wall of the tube. Fill the tube to the 14 ml mark. Cap your tube and let stand undisturbed for 5 minutes at room temperature. What do you see? (You should begin to see bubbles and white strands appearing at the interface between the alcohol and water phases —this is your DNA!) Very gently tilt the tube on its side and then turn it upright about 10 times until the water and alcohol phases are mixed. Once mixed, the DNA should be fully visible as a “precipitate”.

  17. Step 4: Necklace Assembly In this step, you will assemble your DNA necklace. Place the amulet on your desk. Using a plastic pipet, carefully transfer as much of your DNA precipitate and only as little alcohol as you can into the amulet. The amulet requires approximately 0.5 ml to fill. Screw the cap onto the amulet. Slip the waxed cord through the cap. Your DNA necklace is now complete!

  18. Genes in a Bottle™ Kit Congratulations! You have just created your very own DNA necklace!

  19. How long does the DNA in the necklace last? The DNA in the amulet can last for years. Add more alcohol into the vial if some evaporation occurs. Bulletin 6378 Rev A 13-0498 0313

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