GC setup

2. GC setup

3. 2 ml/min 2 ml/min 2 ml/min 103 ml/min 28 ml/min 28 ml/min 24 ml/min 25 ml/min 100 ml/min Split ratio 25:1 Split ratio 24:2 Split ratio 100:1 2 ml/min 1 ml/min 1 ml/min SPLIT Injections www.kinesis-solutions.co.uk/kinesis_techtips.asp?id=11

4. SPLIT Injections

5. Peak overload www.chem.agilent.com/cag/cabu/capgccols.htm

6. 2 mL/min 2 mL/min 3 mL/min 5 mL/min 1 mL/min 3 mL/min SPLITLESS Injections X www.kinesis-solutions.co.uk/kinesis_techtips.asp?id=11

7. SPLITLESS Injections

8. DIRECT Injections

9. Stationary phase interaction

10. Types of Stationary phases

11. Temperature Program

12. Temperature Program

13. Retention Factor Describes the strength of the interaction of a compound with the stationary phase tR-tM VR-VM k’ = = tM VM 1 < k’ < 10