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Howard Rines 1 , Hedera Porter 1 , Marty Carson 2 , and Jerry Ochocki 2

Introgression of Oat Crown Rust Resistance from Diploid Avena strigosa and Tetraploid Avena murphyi. Howard Rines 1 , Hedera Porter 1 , Marty Carson 2 , and Jerry Ochocki 2 1 USDA-ARS Plant Science Research, and Dept. of Agronomy and Plant Genetics, Univ. of Minnesota

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Howard Rines 1 , Hedera Porter 1 , Marty Carson 2 , and Jerry Ochocki 2

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  1. Introgression of Oat Crown Rust Resistance from Diploid Avena strigosa and Tetraploid Avena murphyi Howard Rines1, Hedera Porter1, Marty Carson2, and Jerry Ochocki2 1USDA-ARS Plant Science Research, and Dept. of Agronomy and Plant Genetics, Univ. of Minnesota 2USDA-ARS Cereal Disease Lab, and Dept. of Plant Pathology, Univ. of Minnesota

  2. Crown Rust on Oat

  3. Crown rust of oat Resistance Past – major genes from A. sterilis (6x), relatively short-lived effectiveness

  4. Crown rust of oat Resistance Past – major genes from A. sterilis (6x), relatively short-lived effectiveness Current – Pc94 from A. strigosa (2x), only effective single gene

  5. Crown rust of oat Resistance Past – major genes from A. sterilis (6x), relatively short-lived effectiveness Current – Pc94 from A. strigosa (2x), only effective single gene Alternatives –Major gene combinations Race non-specific – partial, adult plant, multigenic

  6. Crown rust of oat Resistance Past – major genes from A. sterilis (6x), relatively short-lived effectiveness Current – Pc94 from A. strigosa (2x), only effective single gene Alternatives – Major gene combinations Race non-specific – partial, adult plant, multigenic New sources – A. strigosa (2x) – CI 6954SP A. murphyi (4x) – P12

  7. Rust reaction of the parental lines. ParentsRust susceptibility A. strigosa CI 6954 HR A. murphyi P12 HR A. sativa cv. Ogle S A. sativa cv. Black Mesdag (BM) S

  8. Cross 1

  9. A. strigosa CI6954SP X A. sativa cv. Black Mesdag (2n=2x=14) (2n=6x=42) HR embryo rescueS F1 (2n=1x + 3x=28) S

  10. A. strigosa CI6954SP XA. sativa cv. Black Mesdag (2n=2x=14) (2n=6x=42) HR embryo rescue S F1 (2n=1x + 3x=28) S colchicine fertile sectors self-pollinate C1 (2n=2x + 6x=56) S

  11. A. strigosa CI6954SP XA. sativa cv. Black Mesdag (2n=2x=14) (2n=6x=42) HR embryo rescueS F1 (2n=1x + 3x=28) S colchicine fertile sectors self-pollinate C1 (2n=2x + 6x=56) XA. sativa cv. Ogle S(2n=6x=42) S BC1F1   partial fertile self, (1x + 3x + 3x) highly fertile female (2n=7x=49) S

  12. A. strigosa CI6954SP XA. sativa cv. Black Mesdag (2n=2x=14) (2n=6x=42) HR embryo rescueS F1 (2n=1x + 3x=28) S colchicine fertile sectors self-pollinate C1 (2n=2x + 6x=56) XA. sativa cv. Ogle S(2n=6x=42) S BC1F1   partial fertile self, (1x + 3x + 3x) XA. sativa cv. Ogle highly fertile female (2n=7x=49) (2n=6x=42) SS BC2F1 (2n=6x + 0-7) 18R, 62S

  13. A. strigosa CI6954SP XA. sativa cv. Black Mesdag (2n=2x=14) (2n=6x=42) HR embryo rescue S F1 (2n=1x + 3x=28) S colchicine fertile sectors self-pollinate C1 (2n=2x + 6x=56) XA. sativa cv. Ogle S(2n=6x=42) S BC1F1   partial fertile self, (1x + 3x + 3x) X A. sativa cv. Ogle highly fertile female (2n=7x=49) (2n=6x=42) SS BC2F1 BC1F2 (2n=6x + 0-7) (2n=6x + 0-7)18R, 62S 27R, 130S X

  14. A. strigosa CI6954SP XA. sativa cv. Black Mesdag (2n=2x=14) (2n=6x=42) HR embryo rescue S F1 (2n=1x + 3x=28) S colchicine fertile sectors self-pollinate C1 (2n=2x + 6x=56) XA. sativa cv. Ogle S(2n=6x=42) S BC1F1   partial fertile self, (1x + 3x + 3x) X A. sativa cv. Ogle highly fertile female (2n=7x=49) (2n=6x=42) SS BC2F1 XA. sativa cv. Ogle BC1F2 (2n=6x + 0-7) (2n=6x=42) (2n=6x + 0-7)18R, 62S S 27R, 130SBC3F1 (2n=6x + 0–7) 157R, 170S X

  15. Possible interpretations • Suppressor in Black Mesdag • segregates out in BC1F2, BC2F1, and BC3F1 progeny

  16. A. strigosa CI6954SPXA. sativa cv. Black Mesdag (2n=2x=14) (2n=6x=42) HR embryo rescueS F1 (2n=1x + 3x=28) S colchicine fertile sectors self-pollinate C1 (2n=2x + 6x=56)XA. sativa cv. Ogle S(2n=6x=42) S BC1F1   partial fertile self,(1x + 3x + 3x) X A. sativa cv. Ogle highly fertile female (2n=7x=49) (2n=6x=42) SS BC2F1 BC1F2 (2n=6x + 0-7) (2n=6x + 0-7)18R, 62S 27R, 130S X

  17. Possible interpretations • Suppressor in Black Mesdag • segregates out in BC1F2, BC2F1, and BC3F1 progeny • Precedent: • Pc62 (from A. sterilis) suppressed by Pc38 • Pc94 (from A. strigosa) suppressed by Pc38

  18. Possible interpretations • Suppressor in Black Mesdag • segregates out in BC1F2, BC2F1, and BC3F1 progeny • Precedent: • Pc62 (from A. sterilis) suppressed by Pc38 • Pc94 (from A. strigosa) suppressed by Pc38 • Tested: • Pc94 x Black Mesdag --- R

  19. Possible interpretations • Suppressor in Black Mesdag • segregates out in BC1F2, BC2F1, and BC3F1 progeny • Precedent: • Pc62 (from A. sterilis) suppressed by Pc38 • Pc94 (from A. strigosa) suppressed by Pc38 • Tested: • Pc94 x Black Mesdag --- R • Tested: • New R x Black Mesdag • New R x Pc38

  20. Test cross of resistant A.strigosa/Black Mesdag BC2F2 Cross Ogle-c R S 7 0 8 1 6 3 3 7 7 0 8 0 Plant No.Self R S 12(3) - 1 - - 12(3) - 3 10 0 20(10) - 1 - - 20(10) - 3 9 1 24(3) - 1 7 0 24(3) - 2 9 0 Inoculated at seedling stage with crown rust. Pc 38 Jerry Black (Pc 38) Mesdag R SR SR S - - - - * 0 6 0 7 * 0 2 - - * 0 10 - - * 0 2 0 6 * 0 7 0 5 * Pc 94 0 4 0 11 R,MR *crosses with Black Mesdag 35R 47S 2MR/R 3RSP

  21. Transmission of Introduced Resistance • Past efforts with A. strigosa sources 1. Forsberg and co-workers • Resistance present on an alien addition or substitution chromosome, only female transmission • Used gamma irradiation treatment 2. Aung and Chong -- Resistance on an alien addition chromosome, unstable transmission -- Induced non-homologous pairing using crosses to A. longiglumis CW57

  22. Transmission of Introduced Resistance • Past efforts with A. strigosa sources 1. Forsberg and co-workers • Resistance present on an alien addition or substitution chromosome, only female transmission • Used gamma irradiation treatment 2. Aung and Chong -- Resistance on an alien addition chromosome, unstable transmission -- Induced non-homologous pairing using crosses to A. longiglumis CW57

  23. A. strigosa CI6954SP XA. sativa cv. Black Mesdag (2n=2x=14) (2n=6x=42) HR embryo rescue S F1 (2n=1x + 3x=28) S colchicine fertile sectors self-pollinate C1 (2n=2x + 6x=56) XA. sativa cv. Ogle S(2n=6x=42) S BC1F1   partial fertile self, (1x + 3x + 3x) X A. sativa cv. Ogle highly fertile female (2n=7x=49) (2n=6x=42) SS BC2F1 XA. sativa cv. Ogle BC1F2 (2n=6x + 0-7) (2n=6x=42) (2n=6x + 0-7)18R, 62S S 27R, 130SBC3F1 (2n=6x + 0–7) 157R, 170S X

  24. Reciprocal crosses to Ogle-C of resistant A. strigosa CI 6954SP BC3 derivatives as: BC3 plant female male _ SRMRS RMR 12(1)-3 4 3 0 1 1 0 12(3)-12 4 6 0 6 4 0 15(6)-8 3 6 7 2 1 5 20(5)-19 5 3 0 2 4 0 20(7)-6 4 7 1 4 3 1 20(7)-16 7 5 2 5 4 1 20(10)-7 3 2 0 5 8 0 20(10)-17 3 6 1 1 3 0 20(10)-20 5 5 0 7 3 0 20(12)-1 2 2 6 3 7 0 20(12)-9 73578 0 Total 47 48 22 43 46 7

  25. Buckthorn nursery test of resistant A. strigosa BC3F2 • Plant No.RSR/S • 12(1)-1 39 16 2.4 • 12(1)-7 54 14 3.9 • 12(3)-5 56 15 3.7 • 15(6)-19 2 26 0.1 • 18(5)-4 41 13 3.2 • 18(5)-10 52 19 2.7 • 20(7)-6 51 15 3.4 • 21(6)-6 43 5 8.6 • 23(4)-15 18 0 N/A

  26. Cross 2

  27. A. strigosa CI6954SP XA. murphyi P12 (2n=2x=14) (2n=4x=28) HR colchicine HR C1 (2n=2x + 4x=42) MR

  28. A. strigosa CI6954SP XA. murphyi P12 (2n=2x=14) (2n=4x=28) HR colchicine HR C1X A. sativa cv. Ogle (2n=2x + 4x=42) (2n=6x=42) MRS BC1F1 (2n=1x + 2x +3X=42) S

  29. A. strigosa CI6954SP XA. murphyi P12 (2n=2x=14) (2n=4x=28) HR colchicine HR C1XA. sativa cv. Ogle (2n=2x + 4x=42) (2n=6x=42) MRS BC1F1 X A. sativa cv. Ogle (2n=1x+2x+3X=42) (2n=6x=42) S S BC2F1 (2n=?) R,MR,MS,S

  30. Summary • Oat crown rust resistance in accessions of A.strigosa (2x) and A. murphyi (4x)

  31. Summary • Oat crown rust resistance in accessions of A.strigosa (2x) and A. murphyi (4x) • Resistance suppressed in early generation crosses with A. sativa, but resistant segregants in later generations

  32. Summary • Oat crown rust resistance in accessions of A.strigosa (2x) and A. murphyi (4x) • Resistance suppressed in early generation crosses with A. sativa, but resistant segregants in later generations • Resistance is transmitted equally via male or female. In most cases, transmission appears stable.

  33. Summary • Oat crown rust resistance in accessions of A.strigosa (2x) and A. murphyi (4x) • Resistance suppressed in early generation crosses with A. sativa, but resistant segregants in later generations • Resistance is transmitted equally via male or female. In most cases, transmission appears stable. • Preliminary segregation data suggest most lines have a single major gene.

  34. Summary • Oat crown rust resistance in accessions of A.strigosa (2x) and A. murphyi (4x) • Resistance suppressed in early generation crosses with A. sativa, but resistant segregants in later generations • Resistance is transmitted equally via male or female. In most cases, transmission appears stable. • Preliminary segregation data suggest most lines have a single major gene. • Hypersensitive response of many plants further support single major gene.

  35. Cross 3

  36. A. sativa cv. Ogle XA. murphyi P12 (2n=6x=42) (2n=4x=28) S HR F1 (2n=3x + 2x=35) MS, MR

  37. A. sativa cv. Ogle XA. murphyi P12 (2n=6x=42) (2n=4x=28) S HR F1 (2n=3x + 2x=35) MS, MR colchicine fertile sectors self-pollinate F2C1 (?x + ?x) (6x + 4x) 2n = ? (2n=10x=70) S S X

  38. A. sativa cv. Ogle XA. murphyi P12 (2n=6x=42) (2n=4x=28) S HR F1 (2n=3x + 2x=35) MS, MR colchicine fertile sectors self-pollinate F2C1 (?x + ?x) (6x + 4x) XA. sativa cv. Ogle 2n = ? (2n=10x=70) (2n=6x=42) S S S BC1F1 (2n= 6x + 2x =56) S X

  39. A. sativa cv. Ogle XA. murphyi P12 (2n=6x=42) (2n=4x=28) S HR F1 (2n=3x + 2x=35) MS, MR colchicine fertile sectors self-pollinate F2C1 (?x + ?x) (6x + 4x) XA. sativa cv. Ogle 2n = ? (2n=10x=70) (2n=6x=42) S S S BC1F1 XA. sativa cv. Ogle (2n= 6x + 2x =56) (2n=6x=42) SS BC2F1 (2n=6x + 0-7) S X

  40. A. sativa cv. Ogle XA. murphyi P12 (2n=6x=42) (2n=4x=28) S HR F1 (2n=3x + 2x=35) MS, MR colchicine fertile sectors self-pollinate F2C1 (?x + ?x) (6x + 4x) XA. sativa cv. Ogle 2n = ?(2n=10x=70) (2n=6x=42) S S S BC1F1XA. sativa cv. Ogle (2n= 6x + 2x =56) (2n=6x=42) S S BC1F2BC2F1 (2n=6x + 2x=56) (2n=6x + 0-7) S, MS S X X

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