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Determine new application spaces in the sequencing platform using visions systems

Determine new application spaces in the sequencing platform using visions systems. Jessica Perez Summer Research Program in Genomics Massachusetts Institute of Technology Summer 2007. Outline. Approach and Goals Vision Systems Potential Application Spaces Demonstrated Application Spaces.

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Determine new application spaces in the sequencing platform using visions systems

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  1. Determine new application spaces in the sequencing platform using visions systems Jessica Perez Summer Research Program in Genomics Massachusetts Institute of Technology Summer 2007

  2. Outline • Approach and Goals • Vision Systems • Potential Application Spaces • Demonstrated Application Spaces

  3. Project Overview • Approach: use Cognex Smart Sensor and Intellect 1.4.0 software as a model to demonstrate the capabilities of vision systems • Goals: • Explore capabilities of software • Look for application spaces • Take application and prove principal

  4. Vision Systems Software Capabilities Lighting Post Processing

  5. Software Capabilities Blob Identification 1D Bar Scanning Flexible Flaw Detection

  6. User Interface Tool Box Viewing Screen Result Table

  7. Blob Identification Lighter Objects FAILED Darker Objects Meet Parameters

  8. 1D Code Reader Numerical Code Read PASS

  9. Flaw Detection Model

  10. Additional Tests Counting Filtering TOOLS Measurement Optical Character Recognition Reader 2D String Reader

  11. Lighting Geometry Filtering Intensity Answer.com

  12. Common Lighting Techniques Bright Field Dark Field Back Lighting From Vision Light Tech

  13. Post Processing MatLab Histogram Filter Before After

  14. Potential Application Spaces Process: Solexa Screening Consumer: Glass Tubes

  15. Process: Solexa Screening • Quality Control Check in the Solexa Sequencing Process Flowcells Clusters

  16. Proof of Principle • Blobs represent clusters of DNA

  17. Consumer : Test Tube Probe Sonication Sonic Waves DNA

  18. Backlighting BEFORE Detection of Dimensions and Shape of Transparent Objects AFTER Images by Keyence-Engineering Knowledge

  19. Setup Components LED Strobe Light Diffuser Magnification Lens

  20. Camera Strobe Light Tubes Mask Setup

  21. Finish Product Tube under Backlighting Distance to be measured

  22. Conclusion • Vision systems have unlimited potential • Implementation of vision systems will improve the efficiency at various stages of the sequencing process.

  23. Technology Development Group Eva Markiewicz Shailendra Yadav Filip Rege Andrew Barry Todd Sparrow Tracey Honan Pat ___ Directors of Education Shawna Young Dedric Carter Eric Lander Broad Institute Acknowledgements

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