1 / 27

EFFECT OF GREEN TEA EXTRACT TO ANGIOGENESIS IN BONE HEALING EXPERIMENTAL STUDY

EFFECT OF GREEN TEA EXTRACT TO ANGIOGENESIS IN BONE HEALING EXPERIMENTAL STUDY. ERI KRISWANTO. Background:. Non-union is a big problem in bone healing Angiogenesis plays important role in bone healing Green tea is one of the strong anti-angiogenesis agent

kylee
Download Presentation

EFFECT OF GREEN TEA EXTRACT TO ANGIOGENESIS IN BONE HEALING EXPERIMENTAL STUDY

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. EFFECT OF GREEN TEA EXTRACT TO ANGIOGENESIS IN BONE HEALINGEXPERIMENTAL STUDY ERI KRISWANTO

  2. Background: • Non-union is a big problem in bone healing • Angiogenesis plays important role in bone healing • Green tea is one of the strong anti-angiogenesis agent • There are no any research that study the effect of green tea to angiogenesis in bone healing

  3. Green Tea (Cameliasinensis) poliphenols in green tea : • flavanol • flavandiol • flavonol • phenolic acid, • catechin ( 70%) :Epigallocatehchin-3-gallate (EGCG) about 67%, epigallocatehchin (EGC), epicatechingallate (ECG), epicatechin (EC), dangallocatechin (Bokuchava, ..et al. 1980), (Kim, S,et,al.2000), (Fasina, G, et,al. 2004).

  4. Bone Healing

  5. Bone Healing in Mice • Inflamation • Soft callus (cartilage)  day 7th • Chondrocytes presents VEGF  Day 10th • Hard callus (bone)  day 14th • Remodelling ( Street, J,. et. al, 2002)

  6. Effect of EGCG to Angiogenesis

  7. Chondrocyte & osteoblast (Hypoxia) HIF VEGF, FGF, dan TGF fracture PDGF PDGFR, ERK 1/2 Pericyte Endotel Inflamation VEGF Green tea (EGCG) FGFR, PI3K, ERK 1/2 Soft callus Survival, vascular permeability, migration, prolifera-tion, vascular guidance Angiogenesis Chondrocyte apoptosis Rekrutmenosteogenic cell Cartilaginous matrixdegradationandremoval Differentiation Matrix production Neo-vessels Hard callus Remodeling ERK ½, PI3 K

  8. Objective • To know the effect of green tea extract to amount of VEGF presenting cells in the callus • To know the effect of green tea extract to amount of vessels in the callus • To know the effect of green tea extract to cross-sectional-area of vessels in the callus

  9. Materials and Method

  10. DESIGN S1 : sample R : randomization K1 : pure water P1 : 15 mg green tea extract P2 : 20 mg green tea extract P3 : 25 mg green tea extract P4 : 30 mg green tea extract Post Test Control Group Design.

  11. Aclitimation ( 1 week) 35 mices with rigth femur fracture P2 P3 P4 K1 P1 25 mg 30 mg Pure water 15 mg 20 mg Day 10th evaluation Callus harversting IHC staining Analyzation

  12. Aclitimation • ( 1 week) • 35 mices with rigth femur fracture P6 P7 P8 K2 P5 25 mg 30 mg Pure water 15 mg 20 mg Day 14th evaluation • Callus harversting HE staining Analyzation

  13. RESULTS

  14. IHC stainingVEGF presenting cells

  15. a,b,c the different superscript shows there is significant difference ( p < 0.05 ) between groups ( Games – Howell)

  16. HE staining- amount of vessels - cross-sectional area of vessels

  17. a,b,c the different superscript shows there is significant difference ( p<0.05 ) between groups ( List Significant Data)

  18. a,b,c the different superscript shows there is significant difference ( p<0.05 ) between groups ( Games – Howell)

  19. DISCUSSION • Green tea or EGCG per orally shows significant inhibition to angiogenesis(Fassina, G, et, al. 2004). • 1.25 % (4,69 mg/ml) of green tea contains 708 µg/ml EGCG per orally will produce 0,1-0,3 µM EGCG in the blood, this dose is equal with concentration of EGCG in human after drinking 2 or 3 cups of green tea (Wang, Z.Y,et,al. 1992). • Drinking 1.25% of green tea significantly inhibit neo-vascularization in mice’s cornea ( Cao Y, Cao R, 1999).

  20. Discussion Drinking 1.25% (4.69 mg/ml) of green tea ad libitum in mice is equal with 15 mg of green tea extract single dose. Results : • Significant decrease of cross-sectional area of vessels in callus was found at minimal dose of 20 mg green tea extract . • Significant decreaseamount of VEGF presenting cells in callus was found at minimal dose of 25 mg green tea extract • Significant decreasesamount of vessels in callus was found at minimal dose of 25 mg green tea extract

  21. Conclusion • Drinking of green tea > 15 mg in mice equal with drinking > 2 or 3 cups of green tea in human will decrease VEGF presenting cells, amount of vessels, and amount of cross-sectional area of vessels in bone healing. • Green tea significantly inhibit angiogenesis in bone healing.

  22. THANK YOU

More Related