Orientation index

1. a HMEC-1 BPAF 0.8 MASMC HUVEC 0.4 * 0 * 4 8 24 ** * ** 1 ** * 0.8 HMEC-1 0.6 BPAF 0.4 MASMC 0.2 HUVEC 0 0 50 100 150 200 300 400 # # # 0h 4h 8h 24h b * 1 HMEC-1 * BPAF * 0.8 MASMC * Orienation HUVEC * 0.6 * * index Orientation index /n) S q * Cos(2 0.4 * * * * 0.2 0 -0.2 Field strengths ( mV/mm) Figure I. Time (a) and voltage (b) dependence of the orientation responses of vascular cells in electric fields. EF of 200mV/mm induced orientation of cells within 4 hours. By 8 hours and 24 hours, although microvascular cells aligned strongly perpendicular to the EF, each of the other cell types showed a more robust response (a). The insert shows the response curves of the cells in different time-points at 200 mV / mm EF strength. *p<0.05 and **p<0.01; #P<0.05-0.000 when compared with corresponding controls(0 hour) after 4, 8, and 24 hours EF exposure, respectively. n= 65-598 from at least two independent experiments. b. Voltage dependence of vascular cells at individual field strengths of 0-400 mV over 5 hours. The number of cells measured for each point are 57~103 , 56~115, 103~116 and 82~114 for HMEC-1, BPAF, MASMC and HUVEC, respectively. *p<0.01-0.0001 when compared with corresponding controls with no EF (0 mV).