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SMASH 2008 Recap: Introducing Citation, Your Hard-Working Lab Assistant

SMASH 2008 Recap: Introducing Citation, Your Hard-Working Lab Assistant. Dean Olson, Ph.D. Director of Customer Support Protasis Corporation. Citation: Push-Button Sample Loader. Chromatography-grade Fluidics Easy-to-use, Web-based Software Built-in Instrument Controller

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SMASH 2008 Recap: Introducing Citation, Your Hard-Working Lab Assistant

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  1. SMASH 2008 Recap:Introducing Citation, Your Hard-Working Lab Assistant Dean Olson, Ph.D. Director of Customer Support Protasis Corporation

  2. Citation: Push-Button Sample Loader • Chromatography-grade Fluidics • Easy-to-use, Web-based Software • Built-in Instrument Controller • Integral Degassing • Field-serviceable Valves • Small-footprint benchtop packaging • Enjoy the reassurance of feedback control, auto-calibration, and system self-maintenance in applications where the robot is not required • Completely upgradeable to full robotic automation

  3. Chromatography grade pumps Field-serviceable valves Integrated feedback control sensors On-board instrument controller Degas, blanket, sparge Protasis One-Minute™ NMR Lite Software

  4. NMR Metabolomics Rapid, Reproducible and Non-Destructive Low Sensitivity MS Metabolomics The size of a plant metabolome is unknown it is estimated at a few thousand constituents High Sensitivity Instrumentation variability LC-MS: problem in RT repeatability Plant metabolomics LC-NMR 10-6 mol LC-UV 10-9 mol 10-12 mol GC-MS LC-MS 10-15 mol LC-LIF 10-19 mol CE-LIF 10-22 mol Sumner et al. Phytochemistry 2003, 62, 817-836.

  5. Total crude extract TOF MS spectra CONTROL 209.1256 209.1256 spiked WOUNDED RANKED LIST OF INDUCED IONS spiked C12H17O3 PCAof total crude extract MS spectra Total crude extract TOF MS spectra CONTROL WOUNDED Data Courtesty of Jean-Luc Wolfender, University of Geneva, Switzerland

  6. Multiple collection triggered by MS CAP-NMR UPLC-TOF-ES-MS of A. thaliana m/z 209.12 (+/- 10 ppm) m/z 209.12 (+/- 0.5 Da) Induced by wounding Data Courtesty of Jean-Luc Wolfender, University of Geneva, Switzerland

  7. 11 8 7 1 5 12 9 10 4 3 2 Standard 50 mg 10 9 7 3,2,8 11,5 4 12 5.5 5.0 4.5 4.0 3.5 3.0 2.5 2.0 1.5 1.0 0.5 Chemical Shift (ppm) CAP-NMR of ion m/z 209 CAP-NMR ca 1 mg NT=5000 Data Courtesty of Jean-Luc Wolfender, University of Geneva, Switzerland

  8. Further example details can be found in… A LC-MS approach is presented for the isolation of minor key plant biomarkers, in view of their characterization by NMR at the microgram scale …using ultra-performance liquid chromatography-time-of-flight mass spectrometry (UPLC-TOF-MS) … then transferred to semi-preparative LC conditions with MS detection … and subsequent characterization by capillary NMR (CapNMR).

  9. Hot Off the Press: Natural Products Workflow Herein we present a method of extract evaluation using HPLC profiling with biological evaluation16 followed by capillary probe NMR spectroscopy/ESMS/UV combined with NMR database (AntiMarin) evaluation, which reduces the crude extract requirement for dereplication to submilligram quantities.17

  10. Full Robotic Automation Automated Push-Button Discrete Sample Loading vs. You pick

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