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Ex. 21: SIM Medium (Sulfur, Indole , Motility)

Ex. 21: SIM Medium (Sulfur, Indole , Motility). Objectives ?. Day 1. Day 2. 1. Sulfur Reduction. Cysteine desulfurase releases H 2 S from S-containing amino acids Thiosulfate reductase releases H 2 S by allowing S 2 O 3 2- to be final electron acceptor in anaerobic respiration

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Ex. 21: SIM Medium (Sulfur, Indole , Motility)

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  1. Ex. 21: SIM Medium(Sulfur, Indole, Motility) Objectives ?

  2. Day 1

  3. Day 2

  4. 1. Sulfur Reduction Cysteine desulfurase releases H2S from S-containing amino acids Thiosulfate reductase releases H2S by allowing S2O32- to be final electron acceptor in anaerobic respiration Ferrous iron salt in medium leads to visible black precipitate (FeS) if H2S is produced

  5. SIM medium after 24h incubation.

  6. 2. Indole Production • See IMViC

  7. Before addition of Kovac’s reagent

  8. 3. Motility Test

  9. SIM Medium Results and Interpretation? Tube 1: Tube 2: The End

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