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Case Studies

Case Studies. Gregory S. Travlos, DVM, DACVP National Institute of Environmental Health Sciences Research Triangle Park, NC 27709 919-541-0653 Travlos@niehs.nih.gov. Cobalt Sulfate Heptahydrate. Cupric Sulfate. m-Chloroaniline. Riddelliine. Methyleugenol. A Diagnostic Problem.

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Case Studies

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  1. Case Studies Gregory S. Travlos, DVM, DACVP National Institute of Environmental Health Sciences Research Triangle Park, NC 27709 919-541-0653 Travlos@niehs.nih.gov

  2. Cobalt Sulfate Heptahydrate

  3. Cupric Sulfate

  4. m-Chloroaniline

  5. Riddelliine

  6. Methyleugenol

  7. A Diagnostic Problem

  8. A Diagnostic Problem • 13-week, dosed-water study of sodium dichromate dihydrate • F344 rats • Females • Untreated control and 1000 mg/L groups • Hematopoietic assessment included: • CBC • Manual Hct - spun microhematocrit

  9. CBC data: female rats, day 23, dosed-water study of Sodium Dichromate Dihydrate Hematology (means) Control 1000 mg/L Hct, manual (%) 48.0 33.4 Hct (%) 47.7 19.5 Hgb (g/dL) 15.9 9.7 RBC (106/µL) 7.82 4.21 MCV (fL) 61.1 46.5 MCHC (g/dL) 33.3 49.6 Retic (%) 2.7 5.1 nRBC (103/µL) 0.00 1.11

  10. CBC data: female rats, day 23, dosed-water study of Sodium Dichromate Dihydrate Hematology (means) Control 1000 mg/L Hct, manual (%) 48.0 33.4 Hct (%) 47.7 19.5 Hgb (g/dL) 15.9 9.7 RBC (106/µL) 7.82 4.21 MCV (fL) 61.1 46.5 MCHC (g/dL) 33.3 49.6 Retic (%) 2.7 5.1 nRBC (103/µL) 0.00 1.11 What’s the morphological classification?

  11. CBC data: female rats, day 23, dosed-water study of Sodium Dichromate Dihydrate Hematology (means) Control 1000 mg/L Hct, manual (%) 48.0 33.4 Hct (%) 47.7 19.5 Hgb (g/dL) 15.9 9.7 RBC (106/µL) 7.82 4.21 MCV (fL) 61.1 46.5 MCHC (g/dL) 33.3 49.6 Retic (%) 2.7 5.1 nRBC (103/µL) 0.00 1.11 What’s the morphological classification? Microcytic, hyperchromic, responsive anemia.

  12. CBC data: female rats, day 23, dosed-water study of Sodium Dichromate Dihydrate Hematology (means) Control 1000 mg/L Hct, manual (%) 48.0 33.4 Hct (%) 47.7 19.5 Hgb (g/dL) 15.9 9.7 RBC (106/µL) 7.82 4.21 MCV (fL) 61.1 46.5 MCHC (g/dL) 33.3 49.6 Retic (%) 2.7 5.1 nRBC (103/µL) 0.00 1.11 What’s the morphological classification? Microcytic, hyperchromic, responsive anemia. Are you sure?

  13. CBC data: female rats, day 23, dosed-water study of Sodium Dichromate Dihydrate Hematology (means) Control 1000 mg/L Hct, manual (%) 48.0 33.4 Hct (%) 47.7 19.5 Hgb (g/dL) 15.9 9.7 RBC (106/µL) 7.82 4.21 MCV (fL) 61.1 46.5 MCHC (g/dL) 33.3 49.6 Retic (%) 2.7 5.1 nRBC (103/µL) 0.00 1.11 Is the response real?

  14. CBC data: female rats, day 23, dosed-water study of Sodium Dichromate Dihydrate Hematology (means) Control 1000 mg/L Hct, manual (%) 48.0 33.4 Hct (%) 47.7 19.5 Hgb (g/dL) 15.9 9.7 RBC (106/µL) 7.82 4.21 MCV (fL) 61.1 46.5 MCHC (g/dL) 33.3 49.6 Retic (%) 2.7 5.1 Retic (103/µL) 210 220 nRBC (103/µL) 0.00 1.11 Now classify the change:

  15. CBC data: female rats, day 23, dosed-water study of Sodium Dichromate Dihydrate Hematology (means) Control 1000 mg/L Hct, manual (%) 48.0 33.4 Hct (%) 47.7 19.5 Hgb (g/dL) 15.9 9.7 RBC (106/µL) 7.82 4.21 MCV (fL) 61.1 46.5 MCHC (g/dL) 33.3 49.6 Retic (%) 2.7 5.1 Retic (103/µL) 210 220 nRBC (103/µL) 0.00 1.11 Now classify the change: Microcytic, hyperchromic, non-responsive anemia.

  16. CBC data: female rats, day 23, dosed-water study of Sodium Dichromate Dihydrate Hematology (means) Control 1000 mg/L Hct, manual (%) 48.0 33.4 Hct (%) 47.7 19.5 Hgb (g/dL) 15.9 9.7 RBC (106/µL) 7.82 4.21 MCV (fL) 61.1 46.5 MCHC (g/dL) 33.3 49.6 Retic (%) 2.7 5.1 Retic (103/µL) 210 220 nRBC (103/µL) 0.00 1.11 Now classify the change: Microcytic, hyperchromic, non-responsive anemia. Are you sure? What would be the potential mechanism?

  17. CBC data: female rats, day 23, dosed-water study of Sodium Dichromate Dihydrate Hematology (means) Control 1000 mg/L Hct, manual (%) 48.0 33.4 Hct (%) 47.7 19.5 Hgb (g/dL) 15.9 9.7 RBC (106/µL) 7.82 4.21 MCV (fL) 61.1 46.5 MCHC (g/dL) 33.3 49.6 MCHC, manual (g/dL) 33.1 29.0 Retic (%) 2.7 5.1 Retic (103/µL) 210 220 nRBC (103/µL) 0.00 1.11

  18. CBC data: female rats, day 23, dosed-water study of Sodium Dichromate Dihydrate Hematology (means) Control 1000 mg/L Hct, manual (%) 48.0 33.4 Hct (%) 47.7 19.5 Hgb (g/dL) 15.9 9.7 RBC (106/µL) 7.82 4.21 MCV (fL) 61.1 46.5 MCHC (g/dL) 33.3 49.6 MCHC, manual (g/dL) 33.1 29.0 Retic (%) 2.7 5.1 Retic (103/µL) 210 220 nRBC (103/µL) 0.00 1.11 Now classify the change:

  19. CBC data: female rats, day 23, dosed-water study of Sodium Dichromate Dihydrate Hematology (means) Control 1000 mg/L Hct, manual (%) 48.0 33.4 Hct (%) 47.7 19.5 Hgb (g/dL) 15.9 9.7 RBC (106/µL) 7.82 4.21 MCV (fL) 61.1 46.5 MCHC (g/dL) 33.3 49.6 MCHC, manual (g/dL) 33.1 29.0 Retic (%) 2.7 5.1 Retic (103/µL) 210 220 nRBC (103/µL) 0.00 1.11 Now classify the change: Automated results: Microcytic, hyperchromic, non-responsive anemia. Manual results: Microcytic, hypochromic, non-responsive anemia

  20. CBC data: female rats, day 23, dosed-water study of Sodium Dichromate Dihydrate Hematology (means) Control 1000 mg/L Hct, manual (%) 48.0 33.4 Hct (%) 47.7 19.5 Hgb (g/dL) 15.9 9.7 RBC (106/µL) 7.82 4.21 MCV (fL) 61.1 46.5 MCHC (g/dL) 33.3 49.6 MCHC, manual (g/dL) 33.1 29.0 Retic (%) 2.7 5.1 Retic (103/µL) 210 220 nRBC (103/µL) 0.00 1.11 Now classify the change: Automated results: Microcytic, hyperchromic, non-responsive anemia. Manual results: Microcytic, hypochromic, non-responsive anemia. Which do you believe?

  21. Sodium Dichromate Dihydrate Control Treated

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