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Supplementary Figure 2. TGF-b induces EGF-like ligands expression in SK-Hep1 and HepG2 cells. SK-Hep1 and HepG2 cells were incubated with TGF-b (2 ng/ml) at the indicated times. Levels of TGFA, HB-EGF and AR transcripts were analyzed by real time PCR.A. SK-Hep1 cells. B. HepG2 cells. Student’s t test C 0 h cells versus TGF-b-treated cells at short times, and TGF-b treated cells at 24 and 48 h versus their respective controls: *p < 0.05, *** p < 0.001.