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Amantadine Resistance A. Hay (MRC National Institute for Medical Research, London)

Amantadine Resistance A. Hay (MRC National Institute for Medical Research, London) VIRGIL Antiviral Training Course London, 3-6 October 2006. (9 subtypes). (16 subtypes). Location of Ser31Asn mutation in the M2 proton channel which causes resistance to amantadine.

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Amantadine Resistance A. Hay (MRC National Institute for Medical Research, London)

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  1. Amantadine Resistance A. Hay (MRC National Institute for Medical Research, London) VIRGIL Antiviral Training Course London, 3-6 October 2006

  2. (9 subtypes) (16 subtypes)

  3. Location of Ser31Asn mutation in the M2 proton channel which causes resistance to amantadine

  4. Amantadine/Rimantadine resistant mutants • Mutations in M2 Channel pore; residues 26,27,30,31,34 • Emerge frequently in vitro and in vivo(30% or higher) • Similar to wt in infectivity, virulence and transmissibility

  5. Emergence of amantadine resistance in human and animal viruses • Pre 1980’s – low incidence; approx. 1% • Mid 1980’s – European swine viruses (sporadic human cases) • 2000 - - avian H5N1,H9N2 (SE Asia) • 2003 - - avian H5N1; (Z,clade 1); human cases H5N3(SE Asia) H7N2(N America) • 2003 - - human H3N2 (China/Hong Kong; worldwide?)

  6. Emergence of amantadine-resistant H3N2 viruses, 1994-2005 Bright et al 2005

  7. ELISA: Effect of rimantadine on expression of HA in MDCK cells infected with rimantadine-sensitive and rimantadine-resistant influenza A H3N2 viruses S31N S31N +Rim A30V +Rim

  8. Sensitivity of H5N1 Viruses to Rimantadine

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