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Johan Enquist, Madeline Ferwerda, Anuradha Madhavan, Derek Hok and Jennifer L Whistler*

Chronic Ethanol Potentiates the Effect of Neuropeptide S in the Basolateral Amygdala and Shows Increased Anxiolytic and Anti-Depressive Effects. Johan Enquist, Madeline Ferwerda, Anuradha Madhavan, Derek Hok and Jennifer L Whistler*. Background. Stress mediator

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Johan Enquist, Madeline Ferwerda, Anuradha Madhavan, Derek Hok and Jennifer L Whistler*

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  1. Chronic Ethanol Potentiates the Effect of Neuropeptide Sin the Basolateral Amygdala and Shows Increased Anxiolyticand Anti-Depressive Effects Johan Enquist, Madeline Ferwerda, Anuradha Madhavan, Derek Hok and Jennifer L Whistler*

  2. Background • Stress mediator --activate arousal and stress-responsive mechanisms -- associated with orexin system and CRF system (Ox1 CRF1) -- suppress anxiety-like behaviors in response to stress

  3. Addiction-related -- effect on the reward system from drug abuse Orexin 1 anta NPSR anta CRF 1 anta

  4. Main Paper Materials and Methods Animals: male, 8 weeks old, mice. Dose of NPS :3 nmol per animal EtOH Consumption: 20% EtOH contingent access for 4 weeks. Anxiety-like tests: Light/dark transition box, Elevated plus maze, Open field test. Depressive-like tests: Forced swim test, Tail suspension test. Conditioned Place Preference(CPP)

  5. Light/dark transition box Elevated plus maze Open field test Forced swim test Tail suspension test Conditioned Place Preference

  6. Elevated plus maze Light/dark transition box Open field test

  7. Forced swim test Tail suspension test

  8. Animal had 1 hrs between the infusion and access to EtOH or Water EtOH Consumption Animal had 4 hrs between the infusion and access to EtOH or Water

  9. Conditioned Place Preference

  10. Whole cell patch-clamp on GABA neurons at BLA

  11. Light/dark transition box Injection of NPS into BLA

  12. Take home message NPS makes you happy. Thank you!

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