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Cellular Respiration: Harvesting Chemical Energy through Aerobic & Anaerobic Metabolism

Explore the process of cellular respiration and how it harvests chemical energy from food, converting it to ATP for cellular work. Learn about the stages of aerobic and anaerobic metabolism, the role of oxygen, and the relationship between cellular respiration and breathing.

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Cellular Respiration: Harvesting Chemical Energy through Aerobic & Anaerobic Metabolism

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  1. Chapter 8 Cellular Respiration: Harvesting Chemical Energy

  2. Aerobic & Anaerobic Metabolism • Aerobic metabolism - When enough oxygen reaches cells to support energy needs - Maximum energy production • Anaerobic metabolism • When the demand for oxygen outstrips the body’s ability to deliver it • Low energy production

  3. AEROBIC HARVEST OF FOOD ENERGY • Cellular respiration is the main way that chemical energy is harvested from food and converted to ATP for cellular work • Cellular respiration is an aerobic process requiring oxygen

  4. The Versatility of Cellular Respiration Cellular respiration can “burn” other kinds of molecules besides glucose: Diverse types of carbohydrates Fats Proteins 4

  5. The Overall Equation for Cellular Respiration • A common fuel molecule for cellular respiration is glucose • This is the overall equation for what happens to glucose during cellular respiration Glucose Oxygen Carbon dioxide Water Energy

  6. But Remember … • Cellular Respiration is a metabolic pathway,not a single reaction • Many chemical reactions, both aerobic and anaerobic, are involved in the process of cellular respiration • Lots of enzymes are required for the process to occur

  7. The Relationship Between Cellular Respiration and Breathing • Cellular respiration and breathing are closely related • Cellular respiration requires a cell to exchange gases with its surroundings • Breathing exchanges these gases between the blood and outside air

  8. The Role of Oxygen in Cellular Respiration • During cellular respiration, hydrogen and its bonding electrons change partners • Hydrogen and its electrons go from sugar to oxygen, forming water

  9. Comparison

  10. The Metabolic Pathway of Cellular Respiration All of the reactions involved in cellular respiration can be grouped into three main stages • Glycolysis – occurs in cytoplasm • The Krebs cycle – occurs in matrix of mitochondria • Electron transport – occurs across the mitochondrial membrane

  11. A Road Map for Cellular Respiration Mitochondrion Cytosol High-energy electrons carried mainly by NADH High-energy electrons carried by NADH Glycolysis Krebs Cycle 2 Pyruvic acid Electron Transport Glucose

  12. Glycolysis Stage One

  13. Stage 1: Glycolysis • Glycolysis takes place in the cytoplasm • Oxygen NOT required • Process breaks a six-carbon glucose into two, three-carbon molecules • A molecule of glucose is split into two molecules of pyruvic acid • These molecules then donate high energy electrons to NAD+, forming NADH

  14. Glycolysis METABOLIC PATHWAY 2 Pyruvic acid Glucose

  15. Glycolysis CoA Acetic acid Pyruvic acid Acetyl-CoA (acetyl-coenzyme A) CO2 Coenzyme A 15

  16. Krebs Cycle Stage Two

  17. Stage 2: The Krebs Cycle • The Krebs cycle completes the breakdown of sugar • It occurs inside the mitochondria • In the Krebs cycle, pyruvic acid from glycolysis is first “prepped” into a usable form by combining it with enzyme Co-A to make Acetyl-CoA

  18. ACETYL Co-A Input Output 2 Acetic acid 1 2 CO2 3 ADP Krebs Cycle 3 NAD 4 FAD 5 6

  19. Electron Transport Stage 3

  20. Stage 3: Electron Transport • Electron transport releases the energy your cells need to make the most of their ATP • The molecules of electron transport chains are built into the inner membranes of mitochondria

  21. Stage 3: Electron Transport The chain functions as a chemical machine that uses energy released by the “fall” of electrons to pump hydrogen ions across the inner mitochondrial membrane These ions store potential energy

  22. Electron transport chain • Cytochromes carry electron carrier molecules (NADH & FADH2) down to oxygen • Chemiosmosis: energy coupling mechanism • ATP synthase: produces ATP by using the H+ gradient (proton-motive force) pumped into the inner membrane space from the electron transport chain; this enzyme harnesses the flow of H+ back into the matrix to phosphorylate ADP to ATP (oxidative phosphorylation)

  23. Protein complex Electron carrier Inner mitochondrial membrane Electron flow ATP synthase Electron transport chain

  24. Food Polysaccharides Fats Proteins Sugars Glycerol Fatty acids Amino acids Amino groups Acetyl- CoA Krebs Cycle Glycolysis Electron Transport

  25. Adding Up the ATP Cytosol Mitochondrion Glycolysis 2 Acetyl- CoA Krebs Cycle 2 Pyruvic acid Electron Transport Glucose Maximum per glucose: by ATP synthase by direct synthesis by direct synthesis Figure 6.14

  26. FERMENTATION: ANAEROBIC HARVEST OF FOOD ENERGY Some of your cells can actually work for short periods without oxygen (anaerobic respiration) For example, muscle cells can produce ATP under anaerobic conditions Called Fermentation Involves The anaerobic harvest of food energy

  27. Fermentation in Human Muscle Cells • Human muscle cells can make ATP with and without oxygen • They have enough ATP to support activities such as quick sprinting for about 5 seconds • A secondary supply of energy (creatine phosphate) can keep muscle cells going for another 10 seconds • To keep running, your muscles must generate ATP by the anaerobic process of fermentation

  28. Glycolysis is the metabolic pathway that provides ATP during fermentation • Pyruvic acid is reduced by NADH, producing NAD+, which keeps glycolysis going • In human muscle cells, lactic acid is a by-product

  29. 2 ADP+ 2 Glycolysis 2 NAD 2 NAD Glucose 2 Pyruvic acid + 2 H 2 Lactic acid Lactic acid fermentation

  30. Fermentation in Microorganisms • Various types of microorganismsperform fermentation • Yeast cells carry out a slightly different type of fermentation pathway • This pathway produces CO2 and ethyl alcohol

  31. 2 ADP+ 2 2 CO2 released 2 ATP Glycolysis 2 NAD 2 NAD 2 Ethyl alcohol Glucose 2 Pyruvic acid + 2 H Alcoholic fermentation

  32. The food industry uses yeast to produce various food products

  33. Related metabolic processes • Fermentation: alcohol~ pyruvate to ethanol lactic acid~ pyruvate to lactate • Facultative anaerobes (yeast/bacteria)

  34. Review: Cellular Respiration • Glycolysis: • 2 ATP (substrate-level phosphorylation) • Kreb’s Cycle: • 2 ATP (substrate-level phosphorylation) • Electron transport & oxidative phosphorylation: • 2 NADH (glycolysis) = 6ATP • 2 NADH (acetyl CoA) = 6ATP • 6 NADH (Kreb’s) = 18 ATP • 2 FADH2 (Kreb’s) = 4 ATP • 38 TOTAL ATP/glucose

  35. Sunlight supplies the energy! Sunlight energy Bonds of Glucose, made in chloroplasts, contain the stored energy Ecosystem Photosynthesis (in chloroplasts) Raw materials for cellular respiration Carbon dioxide Raw materials for photosynthesis Glucose Oxygen Water Glucose broken down to release energy for cellular work Cellular respiration (in mitochondria) Cellular energy Heat energy 36

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