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  1. A B * * * * # * * * # Supplementary Figure 3. Flow cytometry analysis of endothelial cell apoptosis measured by the annexin V-propidium iodide assay (A), and MTT Assay (B). CTRL: Control; D-G: D-Glucose (50 and 100 mM); L-C: L-carnitine (50 and 100 mM); M: Mannitol (50 and 100 mM). Due to the high glucose citotoxicity, at 100 mM cells are completely detached (//). Panel A: * p<0.05 L-C 50, L-C 100 mM and M 50 mM vs CTRL; ** p<0.001 M 100 mM and D-G 50 mM vs CTRL; Panel B: # p<0.01 L-C 100 mM and D-G 50mM vs CTRL. Results are mean  SD values from three independent experiments with different batches of HUVEC.

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