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Ex. 12: Chemical Antimicrobial Agents: Antibiotics

Ex. 12: Chemical Antimicrobial Agents: Antibiotics. Objectives ?? . Fleming and the Discovery of Penicillin . 1928 mold grows on Staphylococcus aureus plate Mold is named Penicillium notatum Florey and Chain isolated Penicillin 10 years later – received Nobel prize. Vocabulary.

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Ex. 12: Chemical Antimicrobial Agents: Antibiotics

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  1. Ex. 12: Chemical Antimicrobial Agents: Antibiotics Objectives ??

  2. Fleming and the Discovery of Penicillin • 1928 mold grows on Staphylococcus aureus plate • Mold is named Penicillium notatum • Florey and Chain isolated Penicillin 10 years later – received Nobel prize

  3. Vocabulary • Antibiotics vs. chemotherapeutic agents • bacteriostatic vs. bactericidal • Commonly used antibiotics: Penicillin, Ampicillin, Tetracycline, Vancomycin, Ciprofloxacin • Agar Disk Diffusion Test or Kirby-Bauer Antimicrobial Susceptibility Test • Mueller-Hinton (MH) agar

  4. Day 1

  5. How to streak for confluent growth Mark the bottom of your plate with a line. This will be your #1 position.

  6. Rotate 60º clockwise

  7. Rotate 30º clockwise Review this 5 mins(in-house) movie clip on how to test for antibiotic susceptibility with the Kirby Bauer method.

  8. Day 2 Examine each plate and look for “zones of inhibition”. Measure  of Zone of inhibition (in mm) and record the value in the table in the lab report section

  9. the end

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