Download
1 / 4
40 likes | 197 Views
CK2 Activity . Beta-dependent CK2 Activity . (P29 Peptide). (eIF2 -derived Peptide). 6000. 20000. 18000. 5000. 16000. 14000. Kinase Activity (cpm/µg) . 4000. Kinase Activity (cpm/µg). 12000. 10000. 3000. 8000. 6000. 2000. 4000. 1000. 2000. 0. siRNA:. 0. Mock. GFP. .
E N D
CK2 Activity Beta-dependent CK2 Activity (P29 Peptide) (eIF2-derived Peptide) 6000 20000 18000 5000 16000 14000 Kinase Activity (cpm/µg) 4000 Kinase Activity (cpm/µg) 12000 10000 3000 8000 6000 2000 4000 1000 2000 0 siRNA: 0 Mock GFP ’ ’ β WT siRNA: Mock GFP ’ ’ WT A siRNA: Fig.S1 Mock GFP CK2 CK2’ CK2’ CK2 WT CK2 CK2’ CK2 Tubulin B
4 2 3 Relative Quantity Relative Quantity 2 1 1 0 0 siRNA: siRNA: Mock GFP CK2 CK2’ CK2' CK2 CK2 CK2’ CK2’ Mock GFP CK2 Snail1 mRNA level 2 3 2 Relative Quantity Relative Quantity 1 1 0 siRNA: CK2 CK2’ CK2’ Mock GFP CK2 C Fig.S1 b CK2 mRNA level CK2’ mRNA level a c d CK2 mRNA level 0 siRNA: CK2 CK2’ CK2’ CK2 Mock GFP
D Fig. S1 Mock CK2’ CK2 GFP CK2 CK2’
E Fig.S1 E-Cadherin / RL-CMV Vimentin / RL-CMV 0.45 0.40 0.35 0.05 0.30 Firefly / Renilla Luciferase Activity Firefly / Renilla Luciferase Activity 0.04 0.25 0.03 0.20 0.15 0.02 0.10 0.01 0.05 0.00 0.00 Mock GFP Mock GFP CK2 CK2’ CK2’ CK2 CK2 CK2’ CK2’ CK2
