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ΕΡΓΑΣΤΗΡΙΑΚΟ ΜΑΘΗΜΑ ΓΕΝΕΤΙΚΗΣ ΚΑΙ ΑΝΟΣΟΛΟΓΙΑΣ

ΕΡΓΑΣΤΗΡΙΑΚΟ ΜΑΘΗΜΑ ΓΕΝΕΤΙΚΗΣ ΚΑΙ ΑΝΟΣΟΛΟΓΙΑΣ. ΑΣΚΗΣΕΙΣ ΔΡΟΣΟΦΙΛΑΣ 2007. Oregon R. female. male. yellow white. Διασταυρώσεις :. Α. ♀ ♀ [yellow body, white eyes] x [wild type] ♂ ♂. ♀ ♀ [yellow body, white eyes, curly wings, scutoid ]

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ΕΡΓΑΣΤΗΡΙΑΚΟ ΜΑΘΗΜΑ ΓΕΝΕΤΙΚΗΣ ΚΑΙ ΑΝΟΣΟΛΟΓΙΑΣ

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  1. ΕΡΓΑΣΤΗΡΙΑΚΟ ΜΑΘΗΜΑ ΓΕΝΕΤΙΚΗΣ ΚΑΙ ΑΝΟΣΟΛΟΓΙΑΣ ΑΣΚΗΣΕΙΣ ΔΡΟΣΟΦΙΛΑΣ 2007

  2. Oregon R female male yellow white

  3. Διασταυρώσεις: Α. ♀♀ [yellow body, white eyes] x [wild type] ♂♂ • ♀♀ [yellow body, white eyes, curly wings, scutoid] • x • [wild type] ♂♂ C. ♀♀ [black body, white eyes, dumpy wings] x [wild type] ♂♂

  4. Διασταυρώσεις: Α. ♀♀ [yellow body, white eyes] x [wild type] ♂♂ • ♀♀ [yellow body, white eyes, curly wings, scutoid] • x • [wild type] ♂♂ C. ♀♀ [black body, white eyes, dumpy wings] x [wild type] ♂♂

  5. Α. ♀♀ [yellow body, white eyes] x [wild type] ♂♂ yw/yw x ++/Y F1 Οι γενετικοί τόποι είναι φυλοσύνδετοι. Τα αλληλόμορφα για το κίτρινο σώμα και τα λευκά μάτια είναι υπολειπόμενα. Αναλογία φύλων: Αρσενικά : Θηλυκά = 130/ 234 = 0,55 Είναι 1:1 ; χ2 = 29.72 df = 1 P < 0.005 ή <0.5%

  6. Α. ♀♀ [wild type (F1)] x [yellow body, white eyes] ♂♂ y w / ++ x y w / Y NR: yw/yw[yw] 1427 NR: yw/Y [yw] NR: ++/yw[++] 2223 NR: ++/Y [++] y w F2 R: y+/yw[y+] 7 R: y+/Y [y+] R: +w/yw[+w] 2 R: +w/Y [+w] + y + + w + or RFobserved = R/Total = 9 / 3659 = 0.00246 0.246 cM RFreal Kosambi = ¼ ln[(1+2RF) / (1-2RF)] = 0.00259  0.259cM y w 0.246 cM

  7. Διασταυρώσεις: Α. ♀♀ [yellow body, white eyes] x [wild type] ♂♂ • ♀♀ [yellow body, white eyes, curly wings, scutoid] • x • [wild type] ♂♂ C. ♀♀ [black body, white eyes, dumpy wings] x [wild type] ♂♂

  8. Sco CyO

  9. ♀♀ [yellow body, white eyes, curly wings, scutoid] x [wild type] ♂♂ • yw/yw ; CyO/Sco X ++/Y ; +/+ F1 yw/++ ; CyO/+ [++CyO] 120 yw/++ ; Sco/+ [++Sco] 183 yw/Y ; CyO/+ [ywCyO] 71 yw/Y ; Sco/+ [ywSco] 93 CyO : Curly of Oster In(2LR)O,Cy,dplvl,pr,cn2 Sco: In(2L)Scorv8 1. Οι γενετικοί τόποι yellow, whiteείναι φυλοσύνδετοι και τα αλληλόμορφά τους υπολειπόμενα. 2. Οι γενετικοί τόποι curly, scutoidείναι αυτοσωμικοί βρίσκονται στο ίδιο χρωμόσωμα και τα αλληλόμορφά τους είναι επικρατή. Αναλογία φύλων: Αρσενικά : Θηλυκά = 164/ 303 =0.54 Είναι 1:1 ; x2 = 41.29 df = 1 P < 0.005 ή <0.5%

  10. B.♀♀[brown body,redeyes,curly wings] x [yellow body,whiteeyes,curlywings,scutoid]♂♂ yw/++ ; CyO/+ x yw/Y ; CyO/Sco F2 RFobserved = R/Total = 45 / 2885 = 0.0156 1.56 cM RFreal Kosambi = ¼ ln[(1+2RF) / (1-2RF)] = 0.0162  1.62cM y w RF(y,w) = 1.5 cM 1.56 cM

  11. B.♀♀[brown body,redeyes,curly wings] x [yellow body,whiteeyes,curlywings,scutoid]♂♂ yw/++ ; CyO/+ x yw/Y ; CyO/Sco Curly 1478 Non Curly 1206 Total 2684 CyO/+ x CyO/Sco CyO/CyO CyO/Sco +/CyO +/Sco ¾ [Curly] ¼ [Non Curly] Αναλογία : Curly: Non Curly = 1478/ 1206 =1.23 Είναι 3:1 ; x2 = 568.75 df = 1 ΑΡΑ: Η αναλογία Curly/Non Curly δεν είναι 3/1! P < 0.005 ή <0.5% CyO/CyO  lethal

  12. Διασταυρώσεις: Α. ♀♀ [yellow body, white eyes] x [wild type] ♂♂ • ♀♀ [yellow body, white eyes, curly wings, scutoid] • x • [wild type] ♂♂ C. ♀♀ [black body, white eyes, dumpy wings] x [wild type] ♂♂

  13. cn bw wt b dp

  14. C. ♀♀ [black body, white eyes, dumpy wings] x [wild type] ♂♂ dp b cnbw / dp b cnbw x + + + + / + + + + F1 +/+ ; dp b cnbw / + + + + [wild type] 86 +/Y ; dp b cnbw / + + + + [wild type] 79 Οι γενετικοί τόποι dumpy, black, cinnabar, brownείναι αυτοσωμικοί και τα αλληλόμορφά τους υπολειπόμενα. Αναλογία φύλων: Αρσενικά : Θηλυκά = 79/ 86=0.92 Είναι 1:1 ; x2 = 0.279 df = 1 0.5 < P < 0.9

  15. C. ♀♀ [wild type] x [black body, white eyes, dumpy wings] ♂♂ dp b cnbw / + + + + x dp b cnbw / dp b cnbw dp b cn bw + + + + dp dp dp + + + dp + + + dp dp + dp cn cn cn cn cn + cn cn + + + + + + bw bw bw bw bw bw bw + + + + + + + + b b b + b b + b + + + + b SR DR TR

  16. C. ♀♀ [wild type] x [black body, white eyes, dumpy wings] ♂♂ dp b cnbw / + + + + x dp b cnbw / dp b cnbw RFdp b = 554/1152=0.481 48.1 cM RFdp cn = 538/1152=0.467 46.7 cM RFdp bw = 545/1152=0.473 47.3 cM RFb cn = 292/1152=0.253 25.3 cM RFb bw = 497/1152=0.431 43.1 cM RFcn bw = 553/1152=0.48 48 cM RFKos (dp b) = 0.986  98.6 cM RFKos (dp cn) = 0.844  84.4 cM RFKos (dp bw) = 0.896  89.6 cM RFKos (b cn) = 0.279  27.9 cM RFKos (b bw) = 0.65  65 cM RFKos (cn bw) = 0.973  97.3 cM ? ?

  17. Chromosome X Chromosome 2 2L 2R

  18. Embryo Stainings engrailed Kruppel 31-1-1 eagled hairy grainyhead pair ruled

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