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Lesson 110

Lesson 110. Learning Goal: (You should be able to…) Develop your microscopy skills : (Can you …) Make scope slides? Use a microscope to make observations? Draw and label elodea cells at various magnifications? Draw and label onion cells at various magnifications?.

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Lesson 110

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  1. Lesson 110 Learning Goal: (You should be able to…) Develop your microscopy skills : (Can you …) Make scope slides? Use a microscope to make observations? Draw and label elodea cells at various magnifications? Draw and label onion cells at various magnifications?

  2. In your Lab note book lesson 110 Drawings of onion cell and elodea cell at various magnifications

  3. Cell organelles Vocabulary vacuole mitochondria lysosome Cell membrane cell wall nucleus ribosome endoplasmic reticulum (ER) cytoplasm chloroplast golgi body Other cell essentials: chemical receptors and messengers enzymes DNA

  4. INSTRUCTIONS & Write To Think 110 • You need the white paper from yesterday and a pencil. Put everything else away in your back pack! • CAREFULLY draw 4 more circles for today’s lesson. • What is the function of chloroplasts? What do they use? • How do you calculate the magnification? • Notice the way the bench is set up and lease make sure its like that when you leave. Thank you!

  5. Skills to work on • Slide preparation • Flat and thin • Air bubbles • Clean and organized • Paper towel • Water, stain and tools • Eye and body position • Focus and scan • Light (if variable) • Drawings and labels

  6. Making a microscope slide of Elodea • Clean • Thin … you only need a small part of one leaf! • Flat … difficult! ‘Tap’ the cover slip with mounted needle • Add a drop or two of water … • BE CAREFULL ABOUT AIR BUBBLES! • Spend time observing at X40, X100 and X400 • Draw some of what you see at TWO magnifications • LABEL THE ORGANELLES!!! • Look for movement of chloroplasts!!!

  7. Making a microscope slide of onion cells • Clean • Thin … you only need a small part! • Carefully peel away the skin of the onion (your teacher might do this for you!) and put it on the slide. • Keep it very flat … difficult! Use the mounted needle and forceps! PATIENCE!! • Add two or three drops of iodine. THIS WILL STAIN YOU and YOUR CLOTHES! • Cover with a coverslip BE CAREFULL ABOUT AIR BUBBLES! • Mop up excess iodine • Spend time observing at X40, X100 and X400 • Draw some of what you see at TWO magnifications • LABEL THE ORGANELLES!!!

  8. See Doc Cam

  9. Clean up • ALL microscope slides in bowl at the back. • PLEASE DO NOT take the cover slips off! • Microscope: • Power off • Stage down • Low (4X) lens vertical • Bench as it was when you came in.

  10. Image of labelled Elodea cell

  11. Picture of labelled onion cell

  12. Questions about onion and elodea cells • Why do we stain onion cells? • Why must the onion cell (specimen) you observe be very thin? • Onion cells are plants. Therefore, why are there no chloroplasts in the onion cells you observed? • What organelles do you suspect are in the Elodea cell? • Did you observe any movement of the any organelles in the cell of Elodea? • Why are the some organelles mostly around the edges of the cell, near to the wall?

  13. Summarizer • Describe the shape and the location of chloroplasts in Elodea cells  • Why were no chloroplasts found in the onion cells? • Which type of cell was smaller - the onion cells or the elodea cells?  • Compare and contrast between the onion cells and the elodea cells. 

  14. End of lesson

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