Bifx Africa-India Joint Virtual Conference 2011

1. Bifx Africa-India Joint Virtual Conference 2011 Bioinformatics for the analysis of hepatitis B virus HBV in pregnant women Silvia Vasquez1 , Howard Fields2 1 Division of Biology, Research and Development Directory, Nuclear Center RACSO, Instituto Peruano de Energía Nuclear, Avenida San Juan de Dios, Km 12 s / n, Carabayllo, Lima, Peru. 2 Division of Viral Hepatitis, Centers for Disease Control and Prevention, Atlanta, USA.

2. Luricocha – Huanta

3. AIM • To analyze phylogenetically sequences that were 402 nucleotide long from the HBsAg gene of HBV obtained from an evaluation of pregnant Peruvian women who received health care in hospitals from Abancay, Huanta or Lima • To identify circulating genotypes

4. HEPATITIS B VIRUS (HBV) • Hepadnaviridae family • 42 nm • Spherical shape • Genome de 3020-3320 nucleotides (string -) • 1700-2800 nucleotides (string +) • Genes: C (core protein) • P (polymerase) • S (pre S1, pre S2, and S) • X ( X protein)

5. HEPATITIS B virions http://es.wikipedia.org/wiki/Hepatitis_B

6. HBV GENOTYPES A and D UNITED STATES and EUROPE B and C ASIA E AFRICA G Low prevalence USA and EUROPE H CALIFORNIA, MEXICO F VENEZUELA, ARGENTINA, BRAZIL PERU

7. Geographical Distribution Chart of the prevalence of HBV in 2005High: prevalence exceeds 8%, Intermediate:2 to 7%, Low: less than 2% http://es.wikipedia.org/wiki/Hepatitis_B

8. MATERIALS AND METHODS • Data Set 16 TAXA • MULTIPLE SEQUENCE ALIGNMENT • CALCULATION OF THE DISTANCE MATRIX • NEIGHBOR-JOINING TREE • BOOTSTRAPING

9. Figura 15. Secuencia de la cepa 131 STRAIN 131 SEQUENCE Stephan Schaefer graph

11. Programas Phylip Joe Felsenstein

12. P-distance matrix (nucleotides) • [ 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 ] • [ 1] • [ 2] 0.055 • [ 3] 0.072 0.067 • [ 4] 0.055 0.060 0.067 • [ 5] 0.060 0.065 0.060 0.037 • [ 6] 0.082 0.075 0.075 0.057 0.062 • [ 7] 0.052 0.072 0.075 0.057 0.060 0.070 • [ 8] 0.082 0.075 0.085 0.060 0.067 0.035 0.072 • [ 9] 0.082 0.077 0.067 0.055 0.060 0.020 0.075 0.045 • [10] 0.072 0.060 0.007 0.065 0.057 0.067 0.072 0.080 0.060 • [11] 0.085 0.080 0.070 0.057 0.062 0.022 0.077 0.047 0.002 0.062 • [12] 0.080 0.070 0.060 0.057 0.057 0.027 0.072 0.052 0.007 0.052 0.010 • [13] 0.080 0.075 0.065 0.052 0.057 0.017 0.072 0.047 0.002 0.057 0.005 0.010 • [14] 0.080 0.080 0.067 0.057 0.062 0.025 0.072 0.045 0.010 0.060 0.012 0.017 0.012 • [15] 0.077 0.072 0.027 0.075 0.070 0.067 0.070 0.080 0.050 0.030 0.052 0.042 0.052 0.050 • [16] 0.062 0.032 0.040 0.052 0.050 0.055 0.065 0.062 0.047 0.032 0.050 0.040 0.045 0.050 0.047

13. Neighbor-joining Phylogenetic tree in peruvian pregnant women

14. Bootstrap Consensus Tree

15. PHYLIP Phylogenetic Tree

16. RESULTS • HBV-A187 Genotype C • HBV-A194 Genotype F • HBV-A163 Genotype F • HBV-HU94 Genotype F • HBV-HU65 Genotype F • HBV-L601 Genotype C • HBV-L29 Genotype F • HBV-L7 Genotype B

17. CONCLUSIONS • The trees obtained from these two methods confirm the same results. • This analysis shows that the sequences from pregnant Peruvian women (8 strains) were homologous with genotypes F (62,5%), C (25%) and B (12,5%). • Genotype F is prevalent in Perú

18. Giving my thanks Carlos Sebastián, Chemical Engineer Fernando Hidalgo, completed doctoral studies in systems engineering, degree in computation Jose Pardo, Statistics and Computer Engineer Katerin Bernal , Computer Engineering Student Richard Osmar León, Computer Engineering Student This group collaborated with the application of Camtasia and I hope this is the begining for a joint collaboration

19. Thank you