Engineering BioBrick vectors from BioBrick parts

Engineering BioBrick vectors from BioBrick parts Reshma P Shetty, Drew Endy and Thomas F Knight Jr

Outline • Background • Synthetic Biology • Terms • Method • Current Research • Discussion

Synthetic Biology • What is Synthetic Biology? • A Method • Milestones • Recombinant DNA • PCR(Polymerase Chain Reaction) • Automated Sequencing • Automated Construction • Standards • Abstractions -Write Last 30 years -------------------Read Synthetic Biology

Synthetic Biology (Cont.) • Systems • Devices • Parts • DNA

Terms • Vector • Plasmid • Polymerase • Restriction enzyme • Ligase

Method • Goal • Standardize the BioBrick vectors’ assembly process • Maintain • Cloning site • Selection marker • High copy orgin • Terminators flanking the cloning site • Verification primer annealing sites • Process • Build new BioBrick vectors’ backbone • Standard assembly for new BioBrick

Method(Cont.) • Basic BioBrick

Method(Cont.) • Build new BioBrick vectors’ backbone

Method(Cont.) • Standard assembly for new BioBrick

Method(Cont.) • Check • Cloning site • Selection marker • High copy orgin • Terminators flanking the cloning site • Verification primer annealing sites

Current Research • More than 200 parts • Some Systems

Discussion • Wetware • Biological film • Digital counting machine • Interdisciplinary research • Computer aided design

Engineering BioBrick vectors from BioBrick parts