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Tao Lin and Jasneet Kaur Lab Advisor: Dr. Edward Bonder Teaching Assistant: Susan Seipel

Tao Lin and Jasneet Kaur Lab Advisor: Dr. Edward Bonder Teaching Assistant: Susan Seipel. RhoA. Rho kinase. mDia. LimK. Myosin phosphatase. MLC-p. MLC. Cofilin. Actin-myosin contractility (cell rounding). EB1/APC. Actin-myosin filament stabilization. Actin polymerization

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Tao Lin and Jasneet Kaur Lab Advisor: Dr. Edward Bonder Teaching Assistant: Susan Seipel

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  1. Tao Lin and Jasneet Kaur Lab Advisor: Dr. Edward Bonder Teaching Assistant: Susan Seipel

  2. RhoA Rho kinase mDia LimK Myosin phosphatase MLC-p MLC Cofilin Actin-myosin contractility (cell rounding) EB1/APC Actin-myosin filament stabilization Actin polymerization Unbranched filaments Stress fibers Microtubule growth and stabilization Is there a correlation between spindle orientation in mitotic cells and the contracted cell shape caused by the GTPase protein, RhoA?

  3. Spread, control cell Contracted cell with constitutively active RhoA Note: These images have been generated through a 90 degree vertical projection of z-series images

  4. Y27632 treatment • Y27632 inhibits Rho kinase which causes actin-myosin contractility leading to rounded cell shape. • Different concentrations of Y27632 used  0μM, 1.0 μM, 1.5 μM, 2.0 μM 2.5 μM, 5.0 μM, 10.0 μM • High concentrations of Y27632 allow the cell shape to return to normal • Goal: What is the point where cell shape remains contracted but the spindle orientation returns to normal? • Will show that the spindle misorientation is not a direct effect of contracted shape caused by RhoA, and thus, is caused by a different aspect of the RhoA signaling pathway.

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