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Nyt ISO dokument

Nyt ISO dokument. Susceptibility testing of infectious agents and evaluation of performance of antimicrobial susceptibility test devices. Part 1: Reference method for testing the in vitro activity of antimicrobial agents against rapidly growing aerobic bacteria involved in infectious diseases.

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Nyt ISO dokument

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  1. Nyt ISO dokument • Susceptibility testing of infectious agents and evaluation of performance of antimicrobial susceptibility test devices. • Part 1: Reference method for testing the in vitro activity of antimicrobial agents against rapidly growing aerobic bacteria involved in infectious diseases.

  2. ISO reference metode • Mikrotitter bouillon fortynding • Cation supplementeret Mueller Hinton bouillon • Inokulat: 5 x 105 CFU/ml (2-8 x 105 CFU/ml) • Inkubations tp: 34-37C, atmosfærisk luft, 18 ± 2 timer.

  3. Intermediate I • bacterial strain that is inhibited in vitro by a concentration of an antimicrobial agent that is associated with uncertain therapeutic effect • NOTE: This class of susceptibility implies that an infection due to the isolate may be appropriately treated in body sites where the drugs are physiologically concentrated or when a high dosage of drug can be used. • NOTE: This class also indicates a “buffer zone” that should prevent small, uncontrolled, technical factors from causing major discrepancies in interpretations.

  4. ISO reference isolater • Staphylococcus aureus ATCC 29213 (NCTC 12973, CIP 103429, DSM 2569) • Enterococcus faecalis ATCC 29212 (NCTC 12697, CIP 103429, DSM 2570) • Escherichia coli ATCC 25922 (NCTC 12241, CIP 7624, DSM 1103) • Pseudomonas aeruginosa ATCC 27853 (NCTC 12973, CIP 54127, DSM 1117) • Streptococcus pneumoniae, ATCC 49619 (NCTC 12977) • Escherichia coli ATCC 35218 (DSM 5564)

  5. ISO reference værdier • Identiske med kendte CLSI / Eucast værdier

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