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微生物應用工業 Ch4. 微生物之發酵代謝

微生物應用工業 Ch4. 微生物之發酵代謝. 阮雪芬 Oct 01, 2002 NTUT. www.ntut.edu.tw/~yukijuan/lectures/Microbio/Oct01.ppt. 碳水化合物的代謝作用. Fig 4-10 EMP pathway: Anaerobic fermentation TCA cycle: Aerobic fermentation. Anaerobic Fermentation. Glycolysis Entner-Doudoroff (ED) pathway Heterolactic fermentation

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微生物應用工業 Ch4. 微生物之發酵代謝

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  1. 微生物應用工業Ch4. 微生物之發酵代謝 阮雪芬 Oct 01, 2002 NTUT www.ntut.edu.tw/~yukijuan/lectures/Microbio/Oct01.ppt

  2. 碳水化合物的代謝作用 • Fig 4-10 • EMP pathway: Anaerobic fermentation • TCA cycle: Aerobic fermentation

  3. Anaerobic Fermentation • Glycolysis • Entner-Doudoroff (ED) pathway • Heterolactic fermentation • Acetone-butanol and butyric acid fermentation • Mixed organic acid fermentation • Hexose-monophosphate shunt (HMP)

  4. Aerobic fermentation • Tricarboxylic acid cycle (TCA cycle) • Glyoxylic acid cycle

  5. Glycolysis • 又稱Embden-Meyerhof-Pernas pathway, 簡稱EMP路徑 (Fig4-11) • 在厭氧條件下,由於微生物所引起之碳水化合物之代謝作用. • 包括: • 酵母的酒精發酵 • 乳酸菌的乳酸發酵 • 大腸菌的發酵

  6. 酒精發酵 • Pyruvic acid CH3HO Ethanol • Pyruvic acid CH3HO Glycerin Dehydrogenase Decarboxylase Na2SO3

  7. 正常乳酸發酵 • Pyruvic acid Lactic acid Lactic dehydrogenase

  8. 解糖作用 基質為肝糖 乳酸為L型 乳酸發酵 葡萄糖 依菌種而不定,有D型,L型及DL型 高等生物的解糖作用與乳酸發酵不同處

  9. Enter-Doudoroff pathway • 簡稱ED pathway • Fig4-13

  10. Heterolactic Fermentation • 乳酸菌利用葡萄糖發酵的結果 • 除生成乳酸之外,尚能伴生乙醇和CO2 • Fig4-14 • 一部份路徑與EMP路徑相同

  11. Acetone-butanol and Butyric Acid Fermentation • Fig 4-16

  12. Hexose-monophosphate shunt (HMP) • Fig4-17 • Fig 4-18 • EMP pathway and HMP pathway, Fig4-19 • Radioactive isotope tracer method

  13. Aerobic Fermentation -Tricarboxylic acid cycle (TCA cycle) • Fig4-22 • 1 Pyruvic acid 3 CO2 + 15 ATP • 1 Glucose 38 ATP (Table 4-4)

  14. Aerobic Fermentation - Glyoxylic acid cycle • The relationship between Glyoxylic acid cycle and TCA cycle (Fig4-23)

  15. 脂質的代謝 • 脂肪脂肪酸 + 甘油 • Fig4-24:脂肪的beta氧化路徑

  16. 其他碳水化合物之代謝作用 • 芳香族化合物之代謝作用 • 以甲皖或甲醇為碳源之氧化途徑: CH4CH3OH HCHO CO2 • C1-化合物之代謝路徑 • 有兩種: • Serine • Allulose • Fig 4-26

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