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Ammonia Oxidizer Enrichment

Ammonia Oxidizer Enrichment. Sarah Douglas Shelbi Russell. Ammonia oxidation. NH 3 + NH 2 OH NO 2 -. HAO. AmoABC. Bacteria. Bacteria & Archaea. Archaeal pathway unknown, hydroxylamine may be oxidized by the nitroxyl pathway (Walker et al., 2010).

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Ammonia Oxidizer Enrichment

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  1. Ammonia Oxidizer Enrichment Sarah Douglas Shelbi Russell

  2. Ammonia oxidation NH3+ NH2OH NO2- HAO AmoABC Bacteria Bacteria & Archaea Archaeal pathway unknown, hydroxylamine may be oxidized by the nitroxyl pathway (Walker et al., 2010)

  3. Phylogenetic diversity of ammonia oxidizers Crenarchaeota Bacteria β-Proteobacteria (Nitrosomonas&Nitrospira) γ-Proteobacteria (Nitrosococcus)

  4. Sampling Monterey Bay Aquarium activated sand 19°C and 20°C Hopkins sand by aquarium outflow and coast

  5. Enrichment procedure • Crenarchaeota medium inoculated with 50 mL of 10 mM filtered slurry water sample • NH4Cl • Cofactors: • 7-vitamin, selenite/tungstate, and SL12 trace metal solution • Bicarbonate carbon source • Phosphate buffer • Store in the dark • Sample every 2 days for 2 weeks

  6. Spectrophotometric determination of substrate and product concentration

  7. Monterey Bay Aquarium Bradley’s experiment Added 200uM Ammonia

  8. PCR amplification of bacterial and archaeal amoA AOA amoA AOB amoA Monterey Bay Aquarium sand 21° Monterey Bay Aquarium sand 18° Hopkins beach sand Hopkins aquarium sand Positive control

  9. Conclusion • Bacterial ammonia oxidizers appear to be more prevalent • Archaea at competitive disadvantage in high ammonia environments? • Lack of correlation between ammonia depletion and nitrite generation: complex ecosystem?

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