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The Lac Operetta

Dive into the whimsical world of molecular biology as RNA polymerase, repressor, and CAP protein sing and dance their way through scenes of glucose, lactose, and cyclic AMP. Join the chorus in this operetta as they navigate the intricate pathways of gene expression, all set to familiar tunes and props inspired by Monty Python, Underdog, and more.

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The Lac Operetta

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  1. The Lac Operetta A musical? travesty with apologies to: Monty Python Underdog Richard Wagner George Gershwin George Frideric Handel Mighty Mouse

  2. Cast of culprits, their tunes & props • RNA polymerase (Underdog song) • Repressor (Lumberjack song, Summertime) • CAP protein (Mighty Mouse song) • Chorus (Ride of the Valkyries) • Lactose (Milky Way bars) • Glucose (Starbursts) • Cyclic AMP (Cinnamon Fire Jolly Ranchers)

  3. Scene 1 – In the glucose • Repressor (bound to the operator): • I'm a repressor and I'm Okay, • I “operate” on DNA. • I keep RNA polymerase away, • Unless I get my Milky Way. • Chorus: • (S)He keeps RNA polymerase away, • Unless (s)he gets his (her) Milky Way.

  4. Scene 1 – Still in the glucose • Enter RNA polymerase (searching along DNA): • Oh, where oh where is my promoter, • Oh, where oh where can it be? • Before I start making RNA • I must on my promoter be. • Chorus: • Before she starts making RNA • She must on her promoter be. • Repressor (blocks RNA polymerase): • I keep RNA polymerase away, • Unless I get my Milky Way!

  5. Scene 2 – In the land of milk and honey (both glucose and lactose) • Repressor (grabbing Milky Way bars, floats away from the DNA): • Milky time, and the lactose is easy. • I've got my lactose, don't care about DNA no mo'..... • RNA polymerase (resumes looking): • Oh, where oh where is my promoter, • Oh, where oh where can it be....?

  6. Scene 3 – Impending Starvation(no glucose) • Chorus (throws cinnamon fire candy (cAMP)): • No more glucose, we are starving, • Send alarms, or else we die! • CAP (catches a couple of red hots, binds to promoter, waves to RNA polymerase): • Have no fear, I'm on my way! • The lac promoter, is right here, • Polymerase, can get in gear! • Polymerize lac RNA!

  7. Scene 3 – triumphant transcription; starvation averted • RNA polymerase transcribes lac RNA • Chorus: • RNA polymerase transcribeth, • Hallelujah, hallelujah, hallelujah, hallelujah! • Ribosomes translate galactosidase, • Hallelujah, hallelujah, hallelujah, hallelujah! • And we shall live for ever and ever! • And we shall live for ever and ever! • Hallelujah!

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