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Single Particle Cryo -EM

Single Particle Cryo -EM. Wen Jiang. http:// jiang.bio.purdue.edu. Markey Center for Structural Biology. Cryo -EM: Imaging & 3 -D Reconstruction. Imaging ( 3D 2D ). Reconstruction ( 2D 3D ). Single Particle Cryo - EM: Jiang Group @ Purdue. West Nile Virus. 20 Å. 10Å. 7 Å. 5Å.

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Single Particle Cryo -EM

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  1. Single Particle Cryo-EM Wen Jiang http://jiang.bio.purdue.edu Markey Center for Structural Biology

  2. Cryo-EM: Imaging & 3-D Reconstruction Imaging (3D2D) Reconstruction(2D3D)

  3. Single Particle Cryo-EM: Jiang Group @ Purdue West Nile Virus 20Å 10Å 7Å 5Å 4Å 3.5Å 3Å T7 capsid I (asym) P22 phage DNA nano-particles T7 capsid I Tulane Virus resolution Circovirus(PCV2) e15 phage (asym) e15phage T7 capsid II

  4. Computing Needs for Cryo-EM Computing Resources Image Processing Tasks Desktop Projection Group of Desktops 2-D Alignment Cluster Condor 3-D Reconstruction ?

  5. http://diagrid.org/tools/cryoem

  6. Cryo-EM on DiaGrid: Benefits • pre-configured installation. As a user, you don’t need to worry about software download and installation. Just focus on your science projects. • rapid dissemination of new algorithms. Newly developed image processing algorithms can be installed here to immediately benefit all user projects. • large computing resource. The computational resources for image processing/3-D reconstruction tasks are provided at no cost to users by DiaGrid which leverages the vast amount of computing resources at Purdue and its partner institutions. http://diagrid.org/tools/cryoem

  7. Demo http://diagrid.org/tools/cryoem

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