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Biotechnology Practice Test. 1. An organism’s chromosomes are part of its ___. Plasmid Recombinant DNA Genome Enzymes. 2. How many microliters will a P-20 pipetter dispense if it reads 005?.
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1. An organism’s chromosomes are part of its ___. • Plasmid • Recombinant DNA • Genome • Enzymes
2. How many microliters will a P-20 pipetter dispense if it reads 005?
3. If you wanted to confirm that you successfully cut a plasmid into several pieces, which of technique would you use? • Ligation • Transformation • Cloning • Gel electrophoresis • Restriction digest
4. What is formed as a result of using restriction enzymes? • Recombinant DNA • Sticky ends • Many copies of a gene • Recombinant plasmid
5. This process puts recombinant plasmids into bacterial cells. • Amplification • Ligation • Restriction digest • Transformation
6. How does a restriction enzyme know where to cut on a piece of DNA? • It finds the right recognition sequence. • It matches complementary bases. • It uses DNA ligase. • It uses gel electrophoresis.
7. The process of making two organisms with the exact DNA is known as ___. • Gene therapy • Cloning • Recombinant technology • Transformation • Ligation
8. Which of the following would be considered a transgenic organism? • A cat that is given a gene that makes dog ears • Human treated with insulin made in bacteria • A sheep that developed from a lab-fertilized egg • A plant grown in a lab from a single plant cell
9. Which of these is FALSE about viruses? • They inject DNA into host cells. • They can replicate recombinant plasmids. • They are used to carry genes into cells. • They trick host cells into making viral proteins.
10. The process that involves inserting a correct gene into a virus then giving that virus to a person is known as ___. • Gene therapy • Cloning • Transformation • Recombinant DNA
11. Why do both sources of DNA in a recombinant have to be cut with the same restriction enzyme?