1 / 20

Kidney Practicals Diuresis

Kidney Practicals Diuresis. Dr. Mona Soliman, MBBS, MSc Department of Physiology College of Medicine 1426 H. Objectives. To measure the volumes and determine the composition of urine excreted by volunteers who have drunk: No fluids (controls) Water Isotonic saline Lasix

lieu
Download Presentation

Kidney Practicals Diuresis

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Kidney PracticalsDiuresis Dr. Mona Soliman, MBBS, MSc Department of Physiology College of Medicine 1426 H

  2. Objectives • To measure the volumes and determine the composition of urine excreted by volunteers who have drunk: • No fluids (controls) • Water • Isotonic saline • Lasix • To be able to discuss the mechanisms by which the body excrete water and sodium

  3. Experimental groups Controls Water Lasix Saline

  4. Methods • Controls • Emptied their bladder at 10:00 am and discard the urine • At 12:00, 12:30, 1:00, 1:30 measured the volumes of urine the produce and provide sample for analysis

  5. Methods…cont. • Water • Emptied their bladder at 10:00 am and discard the urine • At 12:00 drank one liter of water • At 12:00, 12:30, 1:00, 1:30 measured the volumes of urine they produce and provide sample for analysis

  6. Methods…cont. • Saline • Emptied their bladder at 10:00 am and discard the urine • At 12:00 drank one liter of 0.9% saline • At 12:00, 12:30, 1:00, 1:30 measured the volumes of urine they produce and provide sample for analysis

  7. Methods…cont. • Lasix • Emptied their bladder at 10:00 am and discard the urine • At 12:00 swallow lasix tablet (frusemide 40 mg) • At 12:00, 12:30, 1:00, 1:30 measured the volumes of urine they produce and provide sample for analysis

  8. Urine analysis

  9. Methods…cont. • Urine analysis for: • Volume, by a measuring cylinder • [Na+] and [K+] by flame photometry • Osmolality • pH

  10. Calculations

  11. Methods…cont. • Calculations • Flow rate (ml/min) = urine volume (ml)/ time (min) • Total Na excretion (mM)= [Na+] x urine volume (L) • Rate of Na excretion (M/min)= [Na] x flow rate (Divide by 1000 to convert ml to L)

  12. Plot the Graph

  13. Mechanisms???

  14. Deprive of water  Plasma osmolality Stimulates osmoreceptors in hypothalamus  ADH secretion from posterior pituitary Water permeability in late distal tubule and CD  Water reabsorption  Urine osmolarity and  urine volume  Plasma osmolarity toward normal

  15. Drink of water  Plasma osmolality Inhibit osmoreceptors in hypothalamus  ADH secretion from posterior pituitary Water permeability in late distal tubule and CD  Water reabsorption  Urine osmolarity and  urine volume  Plasma osmolarity toward normal

  16. Isotonic Saline (0.9%) • Volume of ECF Osmolality same Stretch on right atrium (volume receptors in right atrium)  ANP (Atrial Natriuretic Peptide)  Na+ excretion by kidneys

  17. ANP Actions • Blood flow to Kidneys (due to relaxation of Smooth muscles of blood Vessels) • GFR • Na+ loss in urine • Aldosterone • Na+ reabsorption in DCT (  Na+ loss in urine)

  18. Lasix (Frusemide 40 mg) Act on thick ascending limb of loop of Henle and blocks the Na+-K+- 2Cl- Co-transport (loop diuretic) • Na+ excretion in urine • water excretion

  19. GOOD LUCK

More Related