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Australian Wagyu Society

Australian Wagyu Society. Geelong October 11, 2013. About GeneSeek. Started in 1998 with one student intern Vision: Affordable, flexible DNA Analysis for agribusiness Now 18 30 43 70 FTE (and many robots) Began 600 sqft , currently 3000 4000 8000 12000

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Australian Wagyu Society

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  1. Australian Wagyu Society Geelong October 11, 2013

  2. About GeneSeek • Started in 1998 with one student intern • Vision: Affordable, flexible DNA Analysis for agribusiness • Now18 3043 70 FTE (and many robots) • Began 600 sqft, currently 3000 40008000 12000 • Acquired by Neogen in 2010 • >700,000 samples this year

  3. Genomic Solutions Custom Content HD SNP Profiling Sample Management Identity & Diagnostics Project Management Tissue/Hair Samples Sequenom Low SNP Sequenom SNP Panels Affymetrix GeneTitan Contract R&D Illumina I-Select Illumina Infinium qPCR Microsatellites Consultation ELISA DNA Rigorous Quality Control

  4. Primary Technology Platforms • Illumina I-Scan - AffymetrixGeneTitan • Higher density SNP profiling (384-1,000,000+) • SequenomMassARRAY • Lower volume SNP panels (1-384) marker assisted selection and Identity Management • DNA Sequencers • Microsatellite panels for identity management and single gene tests for selection and disease management. MySeq for sequence analysis • ELISA and Real-time PCR • Pathogen detection for disease management

  5. High Density SNP Profiling

  6. One Sample, One Stop: Many answers SNP Chips - Informational Powerhouses

  7. One Sample, One Stop: Many answers SNP Chips - Informational Powerhouses Coat Color AM Fertility markers Milk Quality Parentage GGP Fawn Calf

  8. Transforming SNP chips into Informational Powerhouses ? 90K

  9. The “90K” GGP

  10. Dry Matter Intake Birth Weight Mature Height Mature Weight Milk Scrotal Circumference Weaning Weight Yearling Weight Marbling Ribeye Area Fat Thickness Carcass Weight Tenderness Percent Choice (quality grade) Heifer Pregnancy Maternal Calving Ease Direct Calving Ease Docility Average Daily Gain Feed Efficiency Yearling Height Also providing GE-EPDs for Limousin Red Angus Hereford Simmental More pending

  11. Bosindicus • ~1,700 Nellore • ~700 Brahman • ~250 Brangus GGP GGP – Bosindicus 90K – Bosindicus 90K • Commercial heifer product? HD

  12. Why DNA? • Traits that are difficult and/or expensive to measure and have a large economic impact • Carcass traits • Feed Efficiency • Fertility • Immune response • Tenderness • Longevity “ Marker-assisted selection has the potential to greatly improve how quickly we can make genetic progress in many traits for which we do and do not currently have EPDs.” Dr. Mark Enns, Colorado State University June 2009 Brangus Journal

  13. Export markets demand beef that is highly marbled and fat that is very soft. .

  14. Producers in Australia are raising Wagyu cattle to achieve the marbling and soft fat that the Japanese prefer. Wally Ray at the Overflow Ranch in north Queensland.

  15. We tested the effects of different types of ground beef in men. MUFA:SFA = 0.93 MUFA:SFA = 1.25

  16. Ground beef high in MUFA increasesHDL (good) cholesterol in men. MUFA:SFA = 0.93 MUFA:SFA = 1.25

  17. IGENITY® Profile Tenderness Score & WBSF More Tender Shear Force Difference (lb) 2.3 lb. difference IGENITY Profile Tenderness Score Data derived from NBCEC Nov. 2005

  18. Why DNA? • SNP Parentage • Added seedstock value • Multi-sire pastures • Genetic evaluation for commercial cow-calf producers • Solve problems

  19. SNP for parentage and identity Sire B Sire A AT AA TT Calf Sire A is “excluded” as the sire at that locus – except in the case of a genotyping error

  20. Why DNA? • Manage genetic defects Salvage valuable genetics.

  21. Why DNA? When combined with performance records, genomic data improved selection response 20% to 41%, depending on the trait1 1Van Eenennaam, A.L., J.H. van der Werf, and M.E.Goddard. 2010. Value of DNA information for bull selection. 9th World Congress of Genetics Applied to Livestock Production, Leipzip, Germany.

  22. Sample Collection

  23. Endless Possibilities… Affordable Custom Chips: any snp number, any sample size SAME GREAT SERVICE!

  24. Thank you!

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