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Bio-imprinting The advance of recent work

Bio-imprinting The advance of recent work . Mu yangyang. The ENZYME ENGINEERING. Further plan. 1. 2. 3. Paper sharing. Recent developments. Contents. Abstract. Based on Vinyl Acetate. Template : vinyl acetate + R-(-)- mandelic acid.

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Bio-imprinting The advance of recent work

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  1. Bio-imprintingThe advance of recent work Mu yangyang The ENZYME ENGINEERING

  2. Further plan 1 2 3 Paper sharing Recent developments Contents The ENZYME ENGINEERING

  3. Abstract • Based on Vinyl Acetate Template : vinyl acetate + R-(-)-mandelicacid • Based on (+)-Ethyl Mandelate Template : (+)-Ethyl Mandelate Template : (+)-Ethyl Mandelate + 1-phenylethanol The ENZYME ENGINEERING

  4. Project Template : Vinyl Acetate + R-(-)-Mandelic Acid Conditions: Detecting per 2h Results: No products were detected after 12h. The ENZYME ENGINEERING

  5. Project The ENZYME ENGINEERING a. Template : (+)-Ethyl Mandelate Conditions: Detecting per 2h Results: The promising results were abserved.

  6. Project The ENZYME ENGINEERING a. Template : (+)-Ethyl Mandelate Conditions: Detecting per 2h Results: The promising results were abserved.

  7. Hot Tip Results: R-(+)-1-phenylethanol reduced obviously, while the S-(-)-1-phenylethanol barely reacted. The conversion of the substrate was 17.2% and the eep>99%. The ENZYME ENGINEERING

  8. Project The ENZYME ENGINEERING b. Template : (+)-Ethyl Mandelate + (R,S)-1-phenylethanol Results: The results were also inspiring. The conversion of the substrate was 22.7% and the eep>99%.

  9. Comparison The ENZYME ENGINEERING

  10. Further plan 1 2 3 Paper sharing Recent developments Contents The ENZYME ENGINEERING

  11. Further work To verify the hypothesis of bio-imprinting process in accordance with compulsory-order mechanism, the following work should be done. Imprinted with R-(+)-1-phenylethanol Imprinted with S-(-)-1-phenylethanol Imprinted with Vinyl Acetate and R-(+)-1-phenylethanol / S-(-)-1-phenylethanol The ENZYME ENGINEERING

  12. Further plan 1 2 3 Paper sharing Recent developments Contents The ENZYME ENGINEERING

  13. Paper The STYLE of your expression in the paper was quite essential. The ENZYME ENGINEERING

  14. Highlight The E valuesdidn’t depend on the catalytic constants involved in the acylation step. The sensitivity of the enantioselectivity was related to the enantiomericform of the leaving alcohol, indicate that the imprinting effect involves modifications near the active site. The ENZYME ENGINEERING

  15. Paper The ENZYME ENGINEERING

  16. Highlight The ENZYME ENGINEERING Asp and Glu with acidic residues were modified with EDC. Chemical modification did not have a direct effect on chemical groups related to the enzyme activity, but may be altering the percentage or the shape of the open form of the lipase. Combining the immobilization and amination seems to be a very useful tool in the combinatorial biocatalysis, which can change the characteristics of the enzyme like specific activity and enantioselectivity.

  17. Thank You !

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