Supplementary Figure 5 Hori et al.

Dissected Brain Storage and Sectioning Protocol (Illustrated) Dissected out Brain Regions 2-Methylbutane (Wako Pure Chemical Industries Ltd., Osaka) Brain immersed in O.C.T. and 0.1 M PB (containing 20% sucrose) solution To make PB: (68.99 g of NaH2PO4.H20, Merck, was dissolved in final volume of 5 L Liquid Nitrogen OCT block stored at -80ºC Gelatin coated slides made in-house (MATUNAMI MICROSLIDE GLASS SUPERFROST S2442) Sectioning Mounting Sectioning performed at -30ºC Supplementary Figure 5 Hori et al.

Supplementary Figure 5 Hori et al.

Supplementary Figure 5 Hori et al.

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Supplementary Figure 4 Hori et al.

Supplementary Figure 7 Hori et al.

Thiruchelvam et al Supplementary Figure 5

Thiruchelvam et al Supplementary Figure 4

Mertens et al., Supplementary Figure

Supplementary Figure 1 Hori et al.

Supplementary Figure S1 Wang et. al.

Supplementary Figure 1 Lewis, et al.

Nakayama et al. , Supplementary Figure 3

Farmer et al Supplementary Figure 1

Supplementary Figure 1. Suzuki et al.

Yoon et al. Supplementary Figure S4

Supplementary Figure 1 Logan et al

Supplementary Figure S1, Schneider et al

Yang et al. Supplementary Figure S1

Oiva et al., Supplementary Figure.

Supplementary Figure 5. Nikolova et al

Supplementary Figure 1_Van Looy et al.

Sansone et al, Supplementary Figure 2

Gentile et al., Supplementary Figure 6

Supplementary figure 1 Palacios et. al

Supplementary Figure 2 Hori et al.