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Single target LAMP assays

Single target LAMP assays. Assays for quarantine Liriomyza (leaf miners) – L. sativae, L. huidobrensis, L. trifolii – methods to be validated Crude extraction method – amplification in 10-20 minutes Also assays in development for TCDVd, CSNV. L. sativae. Universal LAMP. Ligation

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Single target LAMP assays

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  1. Single target LAMP assays • Assays for quarantine Liriomyza (leaf miners) – L. sativae, L. huidobrensis, L. trifolii – methods to be validated • Crude extraction method – amplification in 10-20 minutes • Also assays in development for TCDVd, CSNV L. sativae

  2. Universal LAMP • Ligation • Blocked probes for exonuclease treatment to remove unligated probe • LAMP • Detection of LAMP products on microarray • Position of spot in LAMP product • Need to digest product before application to array? • Biotin labelling, amount of product to apply to array? • Target-specific spots to be incorporated on an array to be ordered July

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